Proceedings ArticleDOI
Electrowetting-based on-chip sample processing for integrated microfluidics
Richard B. Fair,Vijay Srinivasan,Hong Ren,Philip Y. Paik,Vamsee K. Pamula,Michael G. Pollack +5 more
TLDR
In this article, results and data are reported on key aspects of sample processing protocols performed on-chip in a digital microfluidic lab on-a-chip (LABON-A-chip).Abstract:
In this work, results and data are reported on key aspects of sample processing protocols performed on-chip in a digital microfluidic lab-on-a-chip We report the results of experiments on aspects of sample processing, including on-chip preconcentration and dilution, on-chip sample injection or dispensing, and sample mixing It is shown that high speed transport and mixing of analytes and reagents can be performed using biological solutions without system contaminationread more
Citations
More filters
Patent
Amplification d'acides nucléiques
Chieh-Yuan Li,David Ruff,Shiaw-Min Chen,Jennifer O'neil,Rachel Kasinskas,Jonathan M. Rothberg,Wolfgang Hinz +6 more
TL;DR: In this paper, the authors present, dans certains modes de realisation, des procedes d'amplification d'acides nucleiques comprenant the formation of un melange reactionnel and the soumission of l'acide nucleique a amplifier a des conditions de denaturation partielle.
Concepts for Micro-Pneumatic and Micro-Hydraulic Logic Gates
TL;DR: In this article, the steady-state behavior of micro-pneumatic logic gates has been studied and the transfer characteristic for switching between pneumatic states has been derived, and a mathematical treatment of the transient response has been provided.
Book ChapterDOI
Biochip Architecture Model
TL;DR: This chapter presents in detail how digital microfluidic biochips work, and introduces the architecture model the authors use in the book, and presents the key ideas behind electrowetting-on-dielectric, the fluid propulsion method used in theseBiochips.
Patent
Method of growing cells on a droplet actuator
TL;DR: In this article, a method of growing cells on a droplet actuator is described, where the droplet is used to provide a medium for the cells to grow in the cell culture medium on the actuator.
Patent
Assay for B-galactosidase activity
TL;DR: In this paper, an assay for acid β-galactosidase activity was proposed, which may include combining in oil a sample droplet with a 4-methylumbelliferyl-B-Galactose to yield a reaction droplet, splitting the reaction droplets to yield first daughter and second daughter droplets, combining the first daughter droplet and a stop buffer droplet to yield the first stopped reaction, incubating the second daughter, and measuring 4methylUMBelliferone released in the first and second stopped reaction.
References
More filters
Journal ArticleDOI
Electrowetting-based actuation of liquid droplets for microfluidic applications
TL;DR: In this article, a microactuator for rapid manipulation of discrete microdroplets is presented, which is accomplished by direct electrical control of the surface tension through two sets of opposing planar electrodes fabricated on glass.
Journal ArticleDOI
Light-driven motion of liquids on a photoresponsive surface
TL;DR: The light-driven motion of a fluid substance in a surface-modified glass tube suggests potential applicability to microscale chemical process systems.
Journal ArticleDOI
Electrochemical Principles for Active Control of Liquids on Submillimeter Scales
Benedict S. Gallardo,Vinay Gupta,Franklin D. Eagerton,Lana I. Jong,Vincent S. J. Craig,Rahul R. Shah,Nicholas L. Abbott +6 more
TL;DR: Electrochemical methods were combined with redox-active surfactants to actively control the motions and positions of aqueous and organic liquids on millimeter and smaller scales to guide droplets of organic liquids through simple fluidic networks.
Journal ArticleDOI
Dielectrophoretic liquid actuation and nanodroplet formation
TL;DR: In this article, the authors demonstrate a new class of high-speed DEP actuators, including wallless flowstructures, siphons, and nanodroplet dispensers that operate with water.
Journal ArticleDOI
Electrostatic actuation of liquid droplets for micro-reactor applications
TL;DR: In this paper, a device for the electrostatic actuation of liquid droplets on a solid surface is developed, where arrays of electrodes are micro-fabricated on a substrate, which is covered by a hydrophobic layer.