Proceedings ArticleDOI
Electrowetting-based on-chip sample processing for integrated microfluidics
Richard B. Fair,Vijay Srinivasan,Hong Ren,Philip Y. Paik,Vamsee K. Pamula,Michael G. Pollack +5 more
TLDR
In this article, results and data are reported on key aspects of sample processing protocols performed on-chip in a digital microfluidic lab on-a-chip (LABON-A-chip).Abstract:
In this work, results and data are reported on key aspects of sample processing protocols performed on-chip in a digital microfluidic lab-on-a-chip We report the results of experiments on aspects of sample processing, including on-chip preconcentration and dilution, on-chip sample injection or dispensing, and sample mixing It is shown that high speed transport and mixing of analytes and reagents can be performed using biological solutions without system contaminationread more
Citations
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Journal ArticleDOI
BioRoute: A Network-Flow-Based Routing Algorithm for the Synthesis of Digital Microfluidic Biochips
TL;DR: The first network-flow-based routing algorithm that can concurrently route a set of noninterfering nets for the droplet routing problem on biochips is presented and is presented as the first polynomial-time algorithm for simultaneous routing and scheduling using the global-routing paths with a negotiation- based routing scheme.
Patent
Droplet actuator devices and methods employing magnetic beads
TL;DR: In this article, a method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance was proposed, where the beads are magnetically immobilized at a location which is at a distance from a target droplet splitting zone.
Patent
Droplet-based washing
TL;DR: In this article, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface, comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step(a) to yield a combined droplet; and (c) conducting a set of droplets comprising: (i) adroplet which is separated from the surface, and (ii
Patent
Nucleic acid amplification
Chieh-Yuan Li,David Ruff,Jennifer O'neil,Rachel Kasinskas,Shiaw-Min Chen,Jonathan M. Rothberg +5 more
TL;DR: In this article, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for NCA, including partially denaturing conditions.
Journal ArticleDOI
Cross-Contamination Avoidance for Droplet Routing in Digital Microfluidic Biochips
Yang Zhao,Krishnendu Chakrabarty +1 more
TL;DR: A droplet-routing method that avoids cross-contamination in the optimization of droplet flow paths and targets disjoint droplet routes and minimizes the number of cells used for droplet routing is proposed.
References
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Journal ArticleDOI
Electrowetting-based actuation of liquid droplets for microfluidic applications
TL;DR: In this article, a microactuator for rapid manipulation of discrete microdroplets is presented, which is accomplished by direct electrical control of the surface tension through two sets of opposing planar electrodes fabricated on glass.
Journal ArticleDOI
Light-driven motion of liquids on a photoresponsive surface
TL;DR: The light-driven motion of a fluid substance in a surface-modified glass tube suggests potential applicability to microscale chemical process systems.
Journal ArticleDOI
Electrochemical Principles for Active Control of Liquids on Submillimeter Scales
Benedict S. Gallardo,Vinay Gupta,Franklin D. Eagerton,Lana I. Jong,Vincent S. J. Craig,Rahul R. Shah,Nicholas L. Abbott +6 more
TL;DR: Electrochemical methods were combined with redox-active surfactants to actively control the motions and positions of aqueous and organic liquids on millimeter and smaller scales to guide droplets of organic liquids through simple fluidic networks.
Journal ArticleDOI
Dielectrophoretic liquid actuation and nanodroplet formation
TL;DR: In this article, the authors demonstrate a new class of high-speed DEP actuators, including wallless flowstructures, siphons, and nanodroplet dispensers that operate with water.
Journal ArticleDOI
Electrostatic actuation of liquid droplets for micro-reactor applications
TL;DR: In this paper, a device for the electrostatic actuation of liquid droplets on a solid surface is developed, where arrays of electrodes are micro-fabricated on a substrate, which is covered by a hydrophobic layer.