Entropic elasticity of lambda-phage DNA
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This article is published in Science.The article was published on 1994-09-09 and is currently open access. It has received 2089 citations till now.read more
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Journal ArticleDOI
Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers.
TL;DR: Repeated stretching and relaxing of the fiber in the absence of egg extract showed that the loss of histone octamers was irreversible, comparable to forces reported for RNA- and DNA-polymerases.
Journal ArticleDOI
Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner
Eric A. Galburt,Stephan W. Grill,Anna Wiedmann,Lucyna Lubkowska,Jason Choy,Eva Nogales,Eva Nogales,Mikhail Kashlev,Carlos Bustamante +8 more
TL;DR: A regulatory mechanism of transcription elongation in eukaryotes is described by which transcription factors modify the mechanical performance of RNAP II, allowing it to operate against higher loads.
Journal ArticleDOI
Mechanics of random fiber networks—a review
TL;DR: A review of the current understanding of the mechanics of random fiber networks, for which the governing functional is the system enthalpy, as well as to molecular networks, in which thermal fluctuations are important.
Journal ArticleDOI
Sacrificial bonds and hidden length: unraveling molecular mesostructures in tough materials.
Georg E. Fantner,Emin Oroudjev,Georg Schitter,Laura S. Golde,Philipp J. Thurner,Marquesa M. Finch,Patricia Turner,Thomas Gutsmann,Daniel E. Morse,Helen G. Hansma,Paul K. Hansma +10 more
TL;DR: By close analysis of the force spectroscopy curves, additional information can be obtained about the molecules and their bonds to the native constructs and how they appear in single molecule forceSpectroscopy measurements are found.
Journal ArticleDOI
Stretching of Single Collapsed DNA Molecules
Christoph G. Baumann,Victor A. Bloomfield,Steven B. Smith,Carlos Bustamante,Michelle D. Wang,Steven M. Block +5 more
TL;DR: The elastic response of single plasmid and lambda phage DNA molecules was probed using optical tweezers at concentrations of trivalent cations that provoked DNA condensation in bulk to observe worm-like chain (WLC) behavior and the transition from WLC behavior to condensation.
References
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Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads
TL;DR: Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution, and the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvatures in DNA.
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Application of the method of phage T4 DNA ligase-catalyzed ring-closure to the study of DNA structure. II. NaCl-dependence of DNA flexibility and helical repeat.
TL;DR: It is demonstrated that it is possible to determine the molar cyclization factor jM from single ligation reactions in which both circular and linear dimer DNA species are formed concurrently from linear monomers.
Journal ArticleDOI
Polymer conformational statistics. III. Modified Gaussian models of stiff chains
M. Fixman,J. Kovac +1 more
TL;DR: In this article, a modified Gaussian probability for intrachain distances was used to account for polymer chain stiffness or excluded volume effects in non-Newtonian viscosity theory.