Entropic elasticity of lambda-phage DNA
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This article is published in Science.The article was published on 1994-09-09 and is currently open access. It has received 2089 citations till now.read more
Citations
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Stepwise unfolding of titin under force-clamp atomic force microscopy
TL;DR: It is shown that under force-clamp conditions, an engineered titin protein elongates in steps because of the unfolding of its modules and that the waiting times to unfold are exponentially distributed.
Journal ArticleDOI
AFM: a versatile tool in biophysics
Andrea Alessandrini,Paolo Facci +1 more
TL;DR: It is hoped that this review can help the reader in appreciating how atomic force microscopy contributes to the current explosive growth of nanobiosciences, where biology, chemistry and physics merge.
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High flexibility of DNA on short length scales probed by atomic force microscopy
Paul A. Wiggins,Thijn van der Heijden,Fernando Moreno-Herrero,Andrew J. Spakowitz,Rob Phillips,Jonathan Widom,Cees Dekker,Philip C Nelson +7 more
TL;DR: This work uses high-resolution atomic force microscopy on individual DNA molecules to obtain a direct measurement of the bending energy function appropriate for scales down to 5 nm, and finds that on short length scales, spontaneous large-angle bends are many times more prevalent than predicted by the WLC model.
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Nucleosomal fluctuations govern the transcription dynamics of RNA polymerase II.
TL;DR: An optical tweezers assay to follow individual Pol II complexes as they transcribe nucleosomal DNA is developed and obtained direct evidence that transcription through a nucleosome involves transfer of the core histones behind the transcribing polymerase via a transient DNA loop.
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Nanospring behaviour of ankyrin repeats
TL;DR: It is reported that tandem ankyrin repeats exhibit tertiary-structure-based elasticity and behave as a linear and fully reversible spring in single-molecule measurements by atomic force microscopy, and an unexpected ability of unfolded repeats to generate force during refolding is observed.
References
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Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads
TL;DR: Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution, and the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvatures in DNA.
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Application of the method of phage T4 DNA ligase-catalyzed ring-closure to the study of DNA structure. II. NaCl-dependence of DNA flexibility and helical repeat.
TL;DR: It is demonstrated that it is possible to determine the molar cyclization factor jM from single ligation reactions in which both circular and linear dimer DNA species are formed concurrently from linear monomers.
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Polymer conformational statistics. III. Modified Gaussian models of stiff chains
M. Fixman,J. Kovac +1 more
TL;DR: In this article, a modified Gaussian probability for intrachain distances was used to account for polymer chain stiffness or excluded volume effects in non-Newtonian viscosity theory.