Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2
TLDR
A role for C-type lectins CLEC2 and CLEC5A in platelet activation and NET formation is reported and blockade of CLEC 5A and TLR2 attenuates inflammation and increases survival of infected mice.Abstract:
Platelet-leukocyte interactions amplify inflammatory reactions, but the underlying mechanism is still unclear. CLEC5A and CLEC2 are spleen tyrosine kinase (Syk)-coupled C-type lectin receptors, abundantly expressed by leukocytes and platelets, respectively. Whereas CLEC5A is a pattern recognition receptor (PRR) to flaviviruses and bacteria, CLEC2 is the receptor for platelet-activating snake venom aggretin. Here we show that dengue virus (DV) activates platelets via CLEC2 to release extracellular vesicles (EVs), including exosomes (EXOs) and microvesicles (MVs). DV-induced EXOs (DV-EXOs) and MVs (DV-MVs) further activate CLEC5A and TLR2 on neutrophils and macrophages, thereby induce neutrophil extracellular trap (NET) formation and proinflammatory cytokine release. Compared to stat1−/− mice, simultaneous blockade of CLEC5A and TLR2 effectively attenuates DV-induced inflammatory response and increases survival rate from 30 to 90%. The identification of critical roles of CLEC2 and CLEC5A/TLR2 in platelet-leukocyte interactions will support the development of novel strategies to treat acute viral infection in the future. Dengue virus (DENV) promotes leukocyte-platelet interactions that contribute to pathogenesis. Here, the authors report a role for C-type lectins CLEC2 and CLEC5A in platelet activation and NET formation and show that blockade of CLEC5A and TLR2 attenuates inflammation and increases survival of infected mice.read more
Citations
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Platelet gene expression and function in patients with COVID-19.
Bhanu Kanth Manne,Frederik Denorme,Elizabeth A. Middleton,Irina Portier,Jesse W. Rowley,Chris Stubben,Aaron C. Petrey,Neal D. Tolley,Li Guo,Mark J. Cody,Andrew S. Weyrich,Christian C. Yost,Matthew T. Rondina,Robert A. Campbell +13 more
TL;DR: It is demonstrated that SARS-CoV-2 infection is associated with platelet hyperreactivity, which may contribute to COVID-19 pathophysiology.
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Platelet activation and platelet-monocyte aggregate formation trigger tissue factor expression in patients with severe COVID-19.
Eugenio D. Hottz,Eugenio D. Hottz,Isaclaudia G. de Azevedo-Quintanilha,Lohanna Palhinha,Lívia Teixeira,Ester A. Barreto,Camila R. R. Pão,Cassia Righy,Sérgio Franco,Thiago Moreno L. Souza,Pedro Kurtz,Fernando A. Bozza,Patrícia T. Bozza +12 more
TL;DR: It is demonstrated that increased platelet activation and platelet-monocyte aggregate formation are observed in severe COVID-19 patients, but not in patients presenting mild CO VID-19 syndrome, and these data shed light on new pathological mechanisms involving plateletactivation and Platelet-dependent monocyte TF expression, which were associated with COVID -19 severity and mortality.
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Detection of Viral Infections by Innate Immunity.
TL;DR: Recent insights into the influence of PRR activation and inflammasomes on viral infections and what this means for the mammalian host are discussed.
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The Era of Thromboinflammation: Platelets Are Dynamic Sensors and Effector Cells During Infectious Diseases.
Li Guo,Matthew T. Rondina +1 more
TL;DR: These evolving roles of platelets under four different infectious pathogen infections are discussed, of which are dengue, malaria, Esterichia coli (E. coli) and staphylococcus aureus S.Aureus, highlighting the complex interplay of these processes with hemostatic and thrombotic pathways.
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Innate immune receptors in platelets and platelet-leukocyte interactions.
Paula Ribeiro Braga Dib,Anna Cecíllia Quirino-Teixeira,Laura Botelho Merij,Mariana Brandi Mendonça Pinheiro,Stephane Vicente Rozini,Fernanda Brandi Andrade,Eugenio D. Hottz +6 more
TL;DR: An increasing body of evidence is compiled showing the participation of platelet innate immune receptors, including PRRs, in infectious diseases, sterile inflammation, and cancer, and how innate immune receptor participate in platelet communication with leukocytes, modulating leukocyte‐mediated inflammation and immune functions is highlighted.
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