Genomics in mammalian cell culture bioprocessing
Reads0
Chats0
TLDR
M mammalian cell culture use in bioprocessing, the importance of obtaining CHO cell line genetic sequences, and the current status of sequencing efforts are described, and future implications of genomic advances are surmised.About:
This article is published in Biotechnology Advances.The article was published on 2012-05-01 and is currently open access. It has received 57 citations till now. The article focuses on the topics: Bioprocess & Genomics.read more
Citations
More filters
Journal ArticleDOI
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome
Nathan E. Lewis,Xin Liu,Yuxiang Li,Harish Nagarajan,George Yerganian,Edward J. O’Brien,Aarash Bordbar,Anne M Roth,Jeffrey Rosenbloom,Chao Bian,Min Xie,Wenbin Chen,Ning Li,Deniz Baycin-Hizal,Haythem Latif,Jochen Förster,Michael J. Betenbaugh,Iman Famili,Xun Xu,Jun Wang,Bernhard O. Palsson +20 more
TL;DR: This analysis identified hamster genes missing in different CHO cell lines, and detected >3.7 million single-nucleotide polymorphisms (SNPs), 551,240 indels and 7,063 copy number variations.
Journal ArticleDOI
Gene expression in Mammalian cells and its applications.
TL;DR: The molecular technologies, expression system and applications of gene expression in mammalian cell lines are made available to create awareness in researchers, starting their career in gene expression related to mammalian cells.
Journal ArticleDOI
Leveraging advances in biology to design biomaterials
TL;DR: By borrowing methods to design experiments and workflows from the bioprocess engineering community, this work outlines a framework for the incorporation of next-generation bioassays into biomaterials design to effectively optimize function while minimizing complexity.
Journal ArticleDOI
CHO microRNA engineering is growing up: Recent successes and future challenges
Vaibhav Jadhav,Matthias Hackl,Aliaksandr Druz,Aliaksandr Druz,Smriti Shridhar,Cheng-Yu Chung,Kelley M. Heffner,David P. Kreil,David P. Kreil,Michael J. Betenbaugh,Joseph Shiloach,Niall Barron,Johannes Grillari,Nicole Borth +13 more
TL;DR: Both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells are discussed, providing an outlook of potential applications of microRNA engineering in production cell lines.
Journal ArticleDOI
Karyotype variation of CHO host cell lines over time in culture characterized by chromosome counting and chromosome painting.
Sabine Vcelar,Vaibhav Jadhav,Michael Melcher,Norbert Auer,Astrid Hrdina,Rebecca Sagmeister,Kelley M. Heffner,Anja Puklowski,Michael J. Betenbaugh,Till Wenger,Friedrich Leisch,Martina Baumann,Nicole Borth +12 more
TL;DR: Using the population distribution of chromosome numbers per cell as well as chromosome painting to quantify the karyotypic variation in several CHO host cell lines revealed a predominant karyotype for each cell line at the start of the experiment, completed by a large number of variants present in each population.
References
More filters
Journal ArticleDOI
Mapping and quantifying mammalian transcriptomes by RNA-Seq.
TL;DR: Although >90% of uniquely mapped reads fell within known exons, the remaining data suggest new and revised gene models, including changed or additional promoters, exons and 3′ untranscribed regions, as well as new candidate microRNA precursors.
Journal ArticleDOI
RNA-Seq: a revolutionary tool for transcriptomics
TL;DR: The RNA-Seq approach to transcriptome profiling that uses deep-sequencing technologies provides a far more precise measurement of levels of transcripts and their isoforms than other methods.
Journal ArticleDOI
Genome sequencing in microfabricated high-density picolitre reactors
Marcel Margulies,Michael Egholm,William E. Altman,Said Attiya,Joel S. Bader,Lisa A. Bemben,Jan Berka,Michael S. Braverman,Yi-Ju Chen,Zhoutao Chen,Scott Dewell,Lei Du,J. M. Fierro,Xavier V. Gomes,Brian C. Godwin,Wen He,Scott Edward Helgesen,Chun Heen Ho,Gerard P. Irzyk,Szilveszter C. Jando,Maria L. I. Alenquer,Thomas P. Jarvie,Kshama B. Jirage,Jong-Bum Kim,James R. Knight,Janna R. Lanza,John H. Leamon,Steven Lefkowitz,Ming Lei,Jing Li,Kenton Lohman,Hong Lu,Vinod Makhijani,Keith Mcdade,Michael P. McKenna,Eugene W. Myers,Elizabeth Nickerson,John Nobile,Ramona Plant,Bernard P. Puc,Michael T. Ronan,George T. Roth,Gary J. Sarkis,Jan Fredrik Simons,John Simpson,Maithreyan Srinivasan,Karrie R. Tartaro,Alexander Tomasz,Kari A. Vogt,Greg A. Volkmer,Shally H. Wang,Yong Wang,Michael P. Weiner,Pengguang Yu,Richard F. Begley,Jonathan M. Rothberg +55 more
TL;DR: A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Journal ArticleDOI
Sequencing technologies-the next generation
TL;DR: A technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments is presented.
Journal ArticleDOI
Next-generation DNA sequencing.
Jay Shendure,Hanlee P. Ji +1 more
TL;DR: Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.
Related Papers (5)
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
Xun Xu,Harish Nagarajan,Nathan E. Lewis,Shengkai Pan,Zhiming Cai,Xin Liu,Wenbin Chen,Min Xie,Wenliang Wang,Stephanie Hammond,Mikael Rørdam Andersen,Norma F. Neff,Benedetto Passarelli,Winston Koh,H. Christina Fan,Jianbin Wang,Yaoting Gui,Kelvin H. Lee,Michael J. Betenbaugh,Michael J. Betenbaugh,Stephen R. Quake,Iman Famili,Bernhard O. Palsson,Jun Wang +23 more