Open AccessJournal Article
Ghrelin degradation by serum and tissue homogenates: identification of the cleavage sites
Carine De Vriese,Françoise Grégoire,Roger Lema Kisoka,Magali Waelbroeck,Patrick Robberecht,Christine Delporte +5 more
TLDR
In this paper, the degradation of ghrelin by rat and human serum, purified commercial enzymes, and tissues homogenates was analyzed by combining HPLC and mass spectrometry.About:
This article is published in Regulatory Peptides.The article was published on 2004-01-01 and is currently open access. It has received 244 citations till now. The article focuses on the topics: Cleavage (embryo).read more
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Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma
Bin Li,Meghan Sedlacek,Indumathi Manoharan,Rathnam Boopathy,Ellen G. Duysen,Patrick Masson,Oksana Lockridge +6 more
TL;DR: It is demonstrated that mice, unlike humans, have substantial amounts of soluble AChE as well as BChE in their plasma, in contrast to mouse, rat, rabbit, horse, cat, and tiger that have high amounts of plasma carboxylesterase.
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Review of human butyrylcholinesterase structure, function, genetic variants, history of use in the clinic, and potential therapeutic uses.
TL;DR: Gene therapy with a vector that sustains expression for a year from a single injection is a promising method for delivering therapeutic quantities of BChE.
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Novel ghrelin assays provide evidence for independent regulation of ghrelin acylation and secretion in healthy young men.
Jianhua Liu,Catherine E. Prudom,Ralf Nass,Suzan S. Pezzoli,Mary C. Oliveri,Michael L. Johnson,Paula P. Veldhuis,David A. Gordon,Andrew D. Howard,Derrick R. Witcher,H. Mario Geysen,Bruce D. Gaylinn,Michael O. Thorner +12 more
TL;DR: Examination of changes in circulating ghrelin and des-acyl gh Relin in response to meals and fasting using newly developed two-site sandwich assays and sample preservation protocols to allow specific detection of full-length forms highlighted the importance of stringent conditions for sample collection and evaluation.
Journal ArticleDOI
A systematic review of the efficacy and safety of fluoridation
TL;DR: The systematic review's research questions relate to the caries-reducing benefits and associated potential health risks of providing fluoride systemically and the use of topical fluoride agents and the hierarchy of study types considered most relevant for answering each of the clinical questions defined in this review was chosen.
Journal ArticleDOI
Ghrelin: a hormone regulating food intake and energy homeostasis.
TL;DR: The effects of ghrelin on the control of energy homeostasis is updated and the molecular mechanisms of gh Relin synthesis, as well as interaction with GHS receptors and signalling are reviewed.
References
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Journal ArticleDOI
A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
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Ghrelin is a growth-hormone-releasing acylated peptide from stomach.
TL;DR: The occurrence of ghrelin in both rat and human indicates that GH release from the pituitary may be regulated not only by hypothalamic GHRH, but also by ghrelIn, a peptide specifically releases GH both in vivo and in vitro.
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Ghrelin induces adiposity in rodents.
TL;DR: It is proposed that ghrelin, in addition to its role in regulating GH secretion, signals the hypothalamus when an increase in metabolic efficiency is necessary, suggesting an involvement in regulation of energy balance.
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Plasma Ghrelin Levels after Diet-Induced Weight Loss or Gastric Bypass Surgery
David E. Cummings,David S. Weigle,R. Scott Frayo,Patricia A. Breen,Marina K. Ma,E. Patchen Dellinger,Jonathan Q. Purnell +6 more
TL;DR: The increase in the plasma ghrelin level with diet-induced weight loss is consistent with the hypothesis that gh Relin has a role in the long-term regulation of body weight.
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A Receptor in Pituitary and Hypothalamus That Functions in Growth Hormone Release
Andrew D. Howard,Scott D. Feighner,Doris F. Cully,Joseph P. Arena,Paul A. Liberator,Charles Rosenblum,Michel J. Hamelin,Donna L. Hreniuk,Oksana C. Palyha,Jennifer W. Anderson,Philip S. Paress,Carmen Diaz,Michael Chou,Ken K. Liu,Karen K. McKee,Sheng-Shung Pong,Lee-Yuh Chaung,Alex Elbrecht,Mike Dashkevicz,Robert P. Heavens,Michael Rigby,Dalip J. S. Sirinathsinghji,Dennis C. Dean,David G. Melillo,Arthur A. Patchett,Ravi P. Nargund,Patrick R. Griffin,Julie A. DeMartino,Sunil Gupta,James M. Schaeffer,Roy G. Smith,Lex H.T. Van der Ploeg +31 more
TL;DR: A heterotrimeric GTP-binding protein (G protein)-coupled receptor (GPC-R) of the pituitary and arcuate ventro-medial and infundibular hypothalamus of swine and humans was cloned and was shown to be the target of the GHSs.