Harnessing recombinase polymerase amplification for rapid multi-gene detection of SARS-CoV-2 in resource-limited settings.
Dounia Cherkaoui,Da Huang,Benjamin S. Miller,Valérian Turbé,Rachel A. McKendry,Rachel A. McKendry +5 more
TLDR
In this paper, a multiplexed, 1-2-step, fast (20-30min) SARS-CoV-2 molecular test using reverse transcription recombinase polymerase amplification was developed to simultaneously detect two conserved targets -the E and RdRP genes.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2021-05-14 and is currently open access. It has received 33 citations till now. The article focuses on the topics: Recombinase Polymerase Amplification & Nucleic acid amplification technique.read more
Citations
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A rapid RNA extraction-free lateral flow assay for molecular point-of-care detection of SARS-CoV-2 augmented by chemical probes
TL;DR: In this paper , a lateral flow strip-based RNA extraction and amplification-free nucleic acid test (NAT) was proposed for rapid diagnosis of positive COVID-19 cases at POC.
Journal ArticleDOI
Point-of-care diagnostics: recent developments in a pandemic age.
Harshit Harpaldas,Siddarth Arumugam,Chelsey Campillo Rodriguez,Bhoomika Ajay Kumar,Vivian Shi,Samuel K. Sia +5 more
TL;DR: In this article, the authors provide an overview of developments in point-of-care (POC) diagnostics during the COVID-19 pandemic, focusing on points of interest to POC researchers and test developers.
Journal ArticleDOI
Isothermal amplification-assisted diagnostics for COVID-19
TL;DR: Isothermal nucleic acid amplification is a simple procedure that quickly and efficiently accumulates nucleic acids sequences at a constant temperature, without the need for sophisticated equipment as mentioned in this paper , which confers high sensitivity and improves screening at the point of need in lowresource settings.
Journal ArticleDOI
Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification
Zhichen Xu,Dongjuan Chen,Tao Li,Jiayu Yan,Jiang Zhu,Ting He,Rui Hu,Ying Li,Yunhuang Yang,Maili Liu +9 more
TL;DR: In this article , the authors proposed a nucleic acid testing platform, named MiCaR, which couples a microfluidic device with CRISPR-Cas12a and multiplex recombinase polymerase amplification.
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Clinical Validation of a Rapid Variant-Proof RT-RPA Assay for the Detection of SARS-CoV-2
Dounia Cherkaoui,Judith Heaney,Da Huang,Matthew Byott,Benjamin S. Miller,Eleni Nastouli,Rachel A. McKendry +6 more
TL;DR: A variant-proof RT-RPA assay that could detect all eleven SARS-CoV-2 lineages tested, including four variants of concern, offers a significantly faster and simpler alternative to RT-PCR, delivering sensitive and specific results with clinical samples.
References
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A new coronavirus associated with human respiratory disease in China.
Fan Wu,Su Zhao,Bin Yu,Yan-Mei Chen,Wen Wang,Zhi gang Song,Yi Hu,Zhao Wu Tao,Jun Hua Tian,Yuan Yuan Pei,Ming Li Yuan,Yu Ling Zhang,Fa Hui Dai,Yi Liu,Qi Min Wang,Jiao Jiao Zheng,Lin Xu,Edward C. Holmes,Edward C. Holmes,Yong-Zhen Zhang,Yong-Zhen Zhang +20 more
TL;DR: Phylogenetic and metagenomic analyses of the complete viral genome of a new coronavirus from the family Coronaviridae reveal that the virus is closely related to a group of SARS-like coronaviruses found in bats in China.
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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
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The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
Alexander E. Gorbalenya,Susan C. Baker,Ralph S. Baric,Raoul J. de Groot,Christian Drosten,Anastasia A. Gulyaeva,Bart L. Haagmans,Chris Lauber,Andrey M. Leontovich,Benjamin W. Neuman,Dmitry Penzar,Stanley Perlman,Leo L.M. Poon,Dmitry V. Samborskiy,Igor A. Sidorov,Isabel Sola,John Ziebuhr +16 more
TL;DR: The independent zoonotic transmission of SARS-CoV and SARS -CoV-2 highlights the need for studying viruses at the species level to complement research focused on individual pathogenic viruses of immediate significance.
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CRISPR-Cas12-based detection of SARS-CoV-2.
James Paul Broughton,Xianding Deng,Guixia Yu,Clare L Fasching,Venice Servellita,Jasmeet Singh,Xin Miao,Jessica Streithorst,Andrea Granados,Alicia Sotomayor-Gonzalez,Kelsey C. Zorn,Allan Gopez,Elaine Hsu,Wei Gu,Steve Miller,Chao Yang Pan,Hugo Guevara,Debra A. Wadford,Janice S. Chen,Charles Y. Chiu +19 more
TL;DR: The CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
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Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases
Yasuyoshi Mori,Tsugunori Notomi +1 more
TL;DR: The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future.
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