HISN3 Mediates Adaptive Response of Chlamydomonas reinhardtii to Excess Nickel
TLDR
A biological role is identified for HISN3 (the gene coding for phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide isomerase) in regulating the response of Chlamydomonas reinhardtii, a unicellular green alga, to Ni toxicity.Abstract:
Investigation of genes for heavy metal [e.g. nickel (Ni) and zinc (Zn)] absorption and detoxification in green algae is of great importance because some of the metals have become one of the major contaminants in the aquatic ecosystem. In plants, overload of heavy metals modifies many aspects of biological processes. However, the mechanisms by which heavy metals exert detrimental effects are not fully understood. The present study identified a biological role for HISN3 (the gene coding for phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide isomerase) in regulating the response of Chlamydomonas reinhardtii, a unicellular green alga, to Ni toxicity. In higher plants, HISN3 encodes an enzyme catalyzing the fourth step in the histidine biosynthesis pathway, but its functional importance is yet to be identified. Transgenic algae overexpressing HISN3 in C. reinhardtii showed high tolerance to excess Ni, with a 48.3-57.4% increase in cell population and moderate histidine accumulation compared with the wild type. HISN3 overexpression improved accumulation of Chl and photosynthesis efficiency, but suppressed Ni-induced generation of reactive oxygen species and lipid peroxides. Interestingly, more Ni and other metals [Zn, iron (Fe), copper (Cu), manganese (Mn) and magnesium (Mg)] were accumulated in HISN3-overexpressing cells than in the wild type. In contrast, RNA interference of HISN3 depressed Ni accumulation but caused cellular sensitivity to Ni. The elevated metal absorption in the HISN3-overexpressing algae implies that the metals can be removed from water media. Thus, our work presents an example for algae genetically designed to improve tolerance to metal toxicity and environmental restoration of metal-contaminated aquatic ecosystems.read more
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Trace metal metabolism in plants.
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Hydrogen Peroxide, Signaling in Disguise during Metal Phytotoxicity
Ann Cuypers,Sophie Hendrix,Rafaela Amaral dos Reis,Stefanie De Smet,Jana Deckers,Heidi Gielen,Marijke Jozefczak,Christophe Loix,Hanne Vercampt,Jaco Vangronsveld,Els Keunen +10 more
TL;DR: Current knowledge on the dual role of ROS in metal-exposed plants is presented, and evidence for a relationship between H2O2 and plant metal tolerance is provided.
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Metal bioremediation by CrMTP4 over-expressing Chlamydomonas reinhardtii in comparison to natural wastewater-tolerant microalgae strains
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Effect of chromium oxide (III) nanoparticles on the production of reactive oxygen species and photosystem II activity in the green alga Chlamydomonas reinhardtii.
Cristina H. da Costa,François Perreault,Abdallah Oukarroum,Silvia Pedroso Melegari,Radovan Popovic,William Gerson Matias +5 more
TL;DR: The metabolism of C. reinhardtii was the most sensitive to Cr2O3-NP after 24h of treatment, revealing a modification of the short-term metabolic response of algae to Cr1N2-NP exposure.
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A cadmium stress-responsive gene AtFC1 confers plant tolerance to cadmium toxicity
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TL;DR: Analyses of the Chlamydomonas genome advance the understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.
Journal ArticleDOI
PHYTOCHELATINS AND METALLOTHIONEINS: Roles in Heavy Metal Detoxification and Homeostasis
TL;DR: Recent advances in understanding the regulation of PC biosynthesis and MT gene expression and the possible roles of PCs and MTs in heavy metal detoxification and homeostasis are reviewed.