Human B1 cells in umbilical cord and adult peripheral blood express the novel phenotype CD20+CD27+CD43+CD70−
Reads0
Chats0
TLDR
Human B1 cells consist of CD20+CD27+CD43+CD70− cells bearing a skewed B cell receptor repertoire, and are present in umbilical cord and adult peripheral blood.Abstract:
B1 cells differ in many ways from conventional B cells, most prominently in the production of natural immunoglobulin, which is vitally important for protection against pathogens. B1 cells have also been implicated in the pathogenesis of autoimmune dyscrasias and malignant diseases. It has been impossible to accurately study B1 cells during health and illness because the nature of human B1 cells has not been successfully defined. This has produced controversy regarding the existence of human B1 cells. Here, we determined the phenotype of human B1 cells by testing sort-purified B cell fractions for three fundamental B1 cell functions based on mouse studies: spontaneous IgM secretion, efficient T cell stimulation, and tonic intracellular signaling. We found that a small population of CD20+CD27+CD43+ cells present in both umbilical cord and adult peripheral blood fulfilled these criteria and expressed a skewed B cell receptor repertoire. These B cells express little or no surface CD69 and CD70, both of which are markedly up-regulated after activation of CD20+CD27−CD43− (naive) and CD20+CD27+CD43− (memory) B cells. This work identifies human B1 cells as CD20+CD27+CD43+CD70−. We determined that the proportion of B1 cells declines with age, which may contribute to disease susceptibility. Identification of human B1 cells provides a foundation for future studies on the nature and role of these cells in human disease.read more
Citations
More filters
Journal ArticleDOI
Causes, consequences, and reversal of immune system aging
TL;DR: The effects of aging on the immune system are manifest at multiple levels that include reduced production of B and T cells in bone marrow and thymus and diminished function of mature lymphocytes in secondary lymphoid tissues, and elderly individuals do not respond to immune challenge as robustly as the young.
Journal ArticleDOI
Role of Bruton's tyrosine kinase in B cells and malignancies
TL;DR: Efficacy of BTK inhibition as a single agent therapy is strong, but resistance may develop, fueling the development of combination therapies that improve clinical responses and highlighting the importance ofBTK inhibition in cancer therapy.
Journal ArticleDOI
Immune responses in neonates
TL;DR: This review focuses mainly on the developmental and functional mechanisms of the human neonatal immune system and the mechanism of innate and adaptive immunity and the role of neutrophils, antigen presenting cells, differences in subclasses of T lymphocytes (Th1, Th2, Tregs and B cells are discussed.
Journal ArticleDOI
Human memory B cells originate from three distinct germinal center-dependent and -independent maturation pathways.
Magdalena A. Berkowska,Gertjan J. Driessen,Vasilis Bikos,Christina Grosserichter-Wagener,Kostas Stamatopoulos,Andrea Cerutti,Bing He,Katharina Biermann,Johan F. Lange,Mirjam van der Burg,Jacques J.M. van Dongen,Menno C. van Zelm +11 more
TL;DR: Light is shed on human germinal center-dependent and -independent B-cell memory formation and new opportunities to study these processes in immunologic diseases are provided.
Journal ArticleDOI
Stereotyped B-cell receptors in one-third of chronic lymphocytic leukemia: a molecular classification with implications for targeted therapies
Andreas Agathangelidis,Nikos Darzentas,Anastasia Hadzidimitriou,Xavier Brochet,Fiona Murray,Xiao-Jie Yan,Zadie Davis,Ellen J. van Gastel-Mol,Cristina Tresoldi,Charles C. Chu,Nicola Cahill,Véronique Giudicelli,Boris Tichy,Lone Bredo Pedersen,Letizia Foroni,Lisa Bonello,Agnieszka Janus,Karin E. Smedby,Achilles Anagnostopoulos,Hélène Merle-Béral,Nikolaos Laoutaris,Gunnar Juliusson,Paola Francia di Celle,Šárka Pospíšilová,Jesper Jurlander,Christian H. Geisler,Athanasios Tsaftaris,Marie-Paule Lefranc,Anton W. Langerak,David Oscier,Nicholas Chiorazzi,Chrysoula Belessi,Frederic Davi,Richard Rosenquist,Paolo Ghia,Kostas Stamatopoulos +35 more
TL;DR: It is documented that CLL may be subdivided into 2 distinct categories: one with stereotyped and the other with nonstereotyped BCRs, at an approximate ratio of 1:2, and evidence suggests a different ontogeny for these 2 categories.
References
More filters
Journal ArticleDOI
Unmutated Ig V(H) genes are associated with a more aggressive form of chronic lymphocytic leukemia.
Terry J. Hamblin,Zadie Davis,Zadie Davis,Anne Gardiner,Anne Gardiner,David G. Oscier,David G. Oscier,Freda K. Stevenson,Freda K. Stevenson +8 more
TL;DR: In this paper, the authors sequenced the Ig V(H) genes of the tumor cells of 84 patients with CLL and correlated their findings with clinical features, finding that the lack of somatic mutation and trisomy 12 was associated with a less favorable prognosis.
Journal ArticleDOI
Ig V gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia.
Rajendra N. Damle,Tarun Wasil,Tarun Wasil,Tarun Wasil,Franco Fais,Franco Fais,Franco Fais,Fabio Ghiotto,Fabio Ghiotto,Fabio Ghiotto,Angelo Valetto,Angelo Valetto,Angelo Valetto,Steven L. Allen,Steven L. Allen,Steven L. Allen,Aby Buchbinder,Aby Buchbinder,Aby Buchbinder,Daniel R. Budman,Daniel R. Budman,Daniel R. Budman,Klaus Dittmar,Klaus Dittmar,Klaus Dittmar,Jonathan E. Kolitz,Jonathan E. Kolitz,Jonathan E. Kolitz,Stuart M. Lichtman,Stuart M. Lichtman,Stuart M. Lichtman,Philip Schulman,Philip Schulman,Philip Schulman,Vincent Vinciguerra,Vincent Vinciguerra,Vincent Vinciguerra,Kanti R. Rai,Kanti R. Rai,Kanti R. Rai,Manlio Ferrarini,Manlio Ferrarini,Manlio Ferrarini,Nicholas Chiorazzi,Nicholas Chiorazzi,Nicholas Chiorazzi +45 more
TL;DR: In this paper, cellular immunophenotypic studies were performed on a cohort of randomly selected IgM(+) B-chronic lymphocytic leukemia (B-CLL) cases for which Ig V(H) and V(L) gene sequences were available.
Journal ArticleDOI
Chronic lymphocytic leukemia.
TL;DR: From the Institute for Medical Research, North Shore–LIJ Health System (N.R.R.), and the Departments of Medicine, North shore University Hospital, Manhasset, N.Y. (K.C., K.R.) and the Dipartimento di Oncologia Clinica e Sperimentale, Universitá di Genova (M.F.).
Journal ArticleDOI
B cell development pathways.
Richard R. Hardy,Kyoko Hayakawa +1 more
TL;DR: Progress in understanding some aspects of this process in the mouse bone marrow is reviewed, focusing on delineation of the earliest stages of commitment, on pre-B cell receptor selection, and B cell tolerance during the immature-to-mature B cell transition.
Journal ArticleDOI
Human Immunoglobulin (Ig)M+IgD+ Peripheral Blood B Cells Expressing the CD27 Cell Surface Antigen Carry Somatically Mutated Variable Region Genes: CD27 as a General Marker for Somatically Mutated (Memory) B Cells
TL;DR: It is reported here that human IgM+IgD+ peripheral blood (PB) B cells expressing the CD27 cell surface antigen carry mutated V genes, in contrast to CD27-negative IgM-only tonsillar germinal center and plasma cells, which may represent a general marker for memory B cells in humans.
Related Papers (5)
The double life of a B-1 cell: self-reactivity selects for protective effector functions
B-1a and B-1b Cells Exhibit Distinct Developmental Requirements and Have Unique Functional Roles in Innate and Adaptive Immunity to S. pneumoniae
Origins and functions of B-1 cells with notes on the role of CD5.
Robert Berland,Henry H. Wortis +1 more