Journal ArticleDOI
Local Ca2+ detection and modulation of synaptic release by astrocytes
Maria Amalia Di Castro,Julien Chuquet,Nicolas Liaudet,Khaleel Bhaukaurally,Mirko Santello,David Bouvier,Pascale Tiret,Andrea Volterra +7 more
TLDR
Evidence is provided that astrocytes are integrated in local synaptic functioning in adult brain through GTP- and inositol-1,4,5-trisphosphate–dependent signaling and is relevant for basal synaptic function.Abstract:
Astrocytes communicate with synapses by means of intracellular calcium ([Ca(2+)](i)) elevations, but local calcium dynamics in astrocytic processes have never been thoroughly investigated. By taking advantage of high-resolution two-photon microscopy, we identify the characteristics of local astrocyte calcium activity in the adult mouse hippocampus. Astrocytic processes showed intense activity, triggered by physiological transmission at neighboring synapses. They encoded synchronous synaptic events generated by sparse action potentials into robust regional (∼12 μm) [Ca(2+)](i) elevations. Unexpectedly, they also sensed spontaneous synaptic events, producing highly confined (∼4 μm), fast (millisecond-scale) miniature Ca(2+) responses. This Ca(2+) activity in astrocytic processes is generated through GTP- and inositol-1,4,5-trisphosphate-dependent signaling and is relevant for basal synaptic function. Thus, buffering astrocyte [Ca(2+)](i) or blocking a receptor mediating local astrocyte Ca(2+) signals decreased synaptic transmission reliability in minimal stimulation experiments. These data provide direct evidence that astrocytes are integrated in local synaptic functioning in adult brain.read more
Citations
More filters
Journal ArticleDOI
IP 3 R2 null mice display a normal acquisition of somatic and neurological development milestones
Sónia Guerra-Gomes,Daniela Cunha-Garcia,Diana S. Nascimento,Sara Duarte-Silva,Eduardo Loureiro-Campos,Vanessa Morais Sardinha,João Filipe Viana,Nuno Sousa,Patrícia Maciel,Luísa Pinto,João Filipe Oliveira,João Filipe Oliveira +11 more
TL;DR: The results show that the inositol 1,4,5-triphosphate receptor type 2 knockout mouse model is a reliable model to study the functional impact of global IP3 R2-dependent astrocytic Ca2+ elevations and that that IP3R2 KO mice display normal motor coordination, strength and neurological reflexes in adulthood.
Journal ArticleDOI
Astroglial Isopotentiality and Calcium-Associated Biomagnetic Field Effects on Cortical Neuronal Coupling.
TL;DR: It is proposed that bioelectric and biomagnetic fields of the astroglial network equalize extracellular local field potentials and associated local magnetic field potentialS in the cortical layers of the brain areas involved in the processing of information, contributing to the adequate and coherent integration of external and internal signals.
Journal ArticleDOI
Cortical Plasticity Induced by Anodal Transcranial Pulsed Current Stimulation Investigated by Combining Two-Photon Imaging and Electrophysiological Recording.
Zengguang Ma,Xiaolang Du,Feifei Wang,Ran Ding,Yuanyuan Li,Aili Liu,Liang Peng Wei,Shaowei Hou,Feng Chen,Qi Hu,Cunle Guo,Qingyan Jiao,Shujing Liu,Bei Fang,Hui Shen +14 more
TL;DR: Investigating the calcium responses in neurons and astrocytes evoked by a-tPCS with different current intensities provides some indication of the mechanisms underlying a- tPCS-induced cortical plasticity, and indicates that the appropriate current intensity should be used in the application of anodal-transcranial pulsed current stimulation.
Proceedings ArticleDOI
Introducing Astrocytes on a Neuromorphic Processor: Synchronization, Local Plasticity and Edge of Chaos
TL;DR: In this article, an end-to-end biophysically plausible compartmental model of an astrocyte that simulates the intracellular activity in response to the synaptic activity in space and time is presented.
Journal ArticleDOI
A statistical method of identifying interactions in neuron-glia systems based on functional multicell Ca2+ imaging.
Ken Nakae,Yuji Ikegaya,Tomoe Ishikawa,Shigeyuki Oba,Hidetoshi Urakubo,Masanori Koyama,Shin Ishii +6 more
TL;DR: The method can elucidate undiscovered functions of neuron–glia systems via maximum-a-posteriori (MAP)-based parameter estimation by developing a generalized linear model (GLM) of a neuron– glia network.
References
More filters
Journal ArticleDOI
Tripartite synapses: astrocytes process and control synaptic information
TL;DR: There is an emerging view, which is reviewed herein, in which brain function actually arises from the coordinated activity of a network comprising both neurons and glia, rather than the classically accepted paradigm that brain function results exclusively from neuronal activity.
Journal ArticleDOI
Control of synapse number by glia.
TL;DR: It is shown that few synapses form in the absence of glial cells and that the fewsynapses that do form are functionally immature, and that CNS synapse number can be profoundly regulated by nonneuronal signals.
Journal ArticleDOI
Long-term potentiation depends on release of d -serine from astrocytes
Christian Henneberger,Thomas Papouin,Thomas Papouin,Stéphane H. R. Oliet,Stéphane H. R. Oliet,Dmitri A. Rusakov +5 more
TL;DR: It is demonstrated that Ca2+-dependent release of d-serine from an astrocyte controls NMDAR-dependent plasticity in many thousands of excitatory synapses nearby.
Journal ArticleDOI
Definition of the Readily Releasable Pool of Vesicles at Hippocampal Synapses
TL;DR: It is found that hypertonic solutions do not act through changes in intracellular calcium, which means that the synaptic release probability depends on the size of the readily releasable pool.
Journal ArticleDOI
Neuronal Synchrony Mediated by Astrocytic Glutamate through Activation of Extrasynaptic NMDA Receptors
TL;DR: The results reveal a distinct mechanism for neuronal excitation and synchrony and highlight a functional link between astrocytic glutamate and extrasynaptic NMDA receptors.