Journal ArticleDOI
Local Ca2+ detection and modulation of synaptic release by astrocytes
Maria Amalia Di Castro,Julien Chuquet,Nicolas Liaudet,Khaleel Bhaukaurally,Mirko Santello,David Bouvier,Pascale Tiret,Andrea Volterra +7 more
TLDR
Evidence is provided that astrocytes are integrated in local synaptic functioning in adult brain through GTP- and inositol-1,4,5-trisphosphate–dependent signaling and is relevant for basal synaptic function.Abstract:
Astrocytes communicate with synapses by means of intracellular calcium ([Ca(2+)](i)) elevations, but local calcium dynamics in astrocytic processes have never been thoroughly investigated. By taking advantage of high-resolution two-photon microscopy, we identify the characteristics of local astrocyte calcium activity in the adult mouse hippocampus. Astrocytic processes showed intense activity, triggered by physiological transmission at neighboring synapses. They encoded synchronous synaptic events generated by sparse action potentials into robust regional (∼12 μm) [Ca(2+)](i) elevations. Unexpectedly, they also sensed spontaneous synaptic events, producing highly confined (∼4 μm), fast (millisecond-scale) miniature Ca(2+) responses. This Ca(2+) activity in astrocytic processes is generated through GTP- and inositol-1,4,5-trisphosphate-dependent signaling and is relevant for basal synaptic function. Thus, buffering astrocyte [Ca(2+)](i) or blocking a receptor mediating local astrocyte Ca(2+) signals decreased synaptic transmission reliability in minimal stimulation experiments. These data provide direct evidence that astrocytes are integrated in local synaptic functioning in adult brain.read more
Citations
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Neuronal activity determines distinct gliotransmitter release from a single astrocyte
Ana Covelo,Alfonso Araque +1 more
TL;DR: It is shown that mouse hippocampal astrocytes activated by endogenous or exogenous stimuli modulate putative single CA3-CA1 hippocampal synapses and can decode neuronal activity and, in response, release distinct gliotransmitters to differentially regulate neurotransmission atPutative single synapses.
Journal ArticleDOI
Review: Astrocytes in Alzheimer's disease and other age-associated dementias: a supporting player with a central role
Claire J. Garwood,Laura E. Ratcliffe,Julie E. Simpson,Paul R. Heath,Paul G. Ince,Stephen B. Wharton +5 more
TL;DR: Enhancing the supportive and neuroprotective components of the astrocyte response has potential translational applications in therapeutic approaches to dementia.
Journal ArticleDOI
Pathophysiology of astroglial purinergic signalling.
TL;DR: This review summarises contemporary knowledge on the role of purinergic receptors (P2Rs) in a variety of diseases in relation to changes of astrocytic functions and nucleotide signalling and highlights specific aspects of reactive astrogliosis.
Journal ArticleDOI
Dysfunctional Calcium and Glutamate Signaling in Striatal Astrocytes from Huntington's Disease Model Mice.
TL;DR: The data suggest that key aspects of altered striatal function associated with HD may be triggered, at least in part, by dysfunctional astrocytes, thereby providing details of an emerging striatal microcircuit mechanism in HD.
Journal ArticleDOI
Dopamine-Evoked Synaptic Regulation in the Nucleus Accumbens Requires Astrocyte Activity.
Michelle Corkrum,Ana Covelo,Justin Lines,Luigi Bellocchio,Marc T. Pisansky,Kelvin Loke,Ruth Quintana,Patrick E. Rothwell,Rafael Luján,Giovanni Marsicano,Giovanni Marsicano,Eduardo D. Martín,Mark J. Thomas,Paulo Kofuji,Alfonso Araque +14 more
TL;DR: It is shown that in freely behaving mice, astrocytes mediate the dopamine- and amphetamine-induced synaptic regulation, revealing a novel cellular pathway in the brain reward system.
References
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Tripartite synapses: astrocytes process and control synaptic information
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Long-term potentiation depends on release of d -serine from astrocytes
Christian Henneberger,Thomas Papouin,Thomas Papouin,Stéphane H. R. Oliet,Stéphane H. R. Oliet,Dmitri A. Rusakov +5 more
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Journal ArticleDOI
Definition of the Readily Releasable Pool of Vesicles at Hippocampal Synapses
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Journal ArticleDOI
Neuronal Synchrony Mediated by Astrocytic Glutamate through Activation of Extrasynaptic NMDA Receptors
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