Meta-analysis of diagnostic performance of serological tests for SARS-CoV-2 antibodies up to 25 April 2020 and public health implications.
Saverio Caini,Federica Bellerba,Federica Corso,Angelica Diaz-Basabe,Gioacchino Natoli,John Paget,Federica Facciotti,Simone Pietro De Angelis,Sara Raimondi,Domenico Palli,Luca Mazzarella,Pier Giuseppe Pelicci,Pier Giuseppe Pelicci,Paolo Vineis,Sara Gandini +14 more
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TLDR
The diagnostic accuracy of SARS-CoV-2 serological tests was reviewed and it was concluded that these tests should be used for prevalence surveys only in hard-hit areas and not in populations with low levels of seroconverted individuals.Abstract:
We reviewed the diagnostic accuracy of SARS-CoV-2 serological tests. Random-effects models yielded a summary sensitivity of 82% for IgM, and 85% for IgG and total antibodies. For specificity, the pooled estimate were 98% for IgM and 99% for IgG and total antibodies. In populations with ≤ 5% of seroconverted individuals, unless the assays have perfect (i.e. 100%) specificity, the positive predictive value would be ≤ 88%. Serological tests should be used for prevalence surveys only in hard-hit areas.read more
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Considerations for diagnostic COVID-19 tests.
Olivier Vandenberg,Olivier Vandenberg,Delphine Martiny,Olivier Rochas,Alex van Belkum,Zisis Kozlakidis +5 more
TL;DR: The crucial role of diagnostic tests during the first global wave of coronavirus disease 2019 (COVID-19) and the technical and implementation problems encountered during the early phase of the pandemic are explored and future directions for the progressive and better use of diagnostics during a possible resurgence of CO VID-19 in future global waves or regional outbreaks are defined.
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SARS-CoV-2 seroprevalence worldwide: a systematic review and meta-analysis.
Ali Rostami,Mahdi Sepidarkish,Mariska M.G. Leeflang,Seyed Mohammad Riahi,Malihe Nourollahpour Shiadeh,Sahar Esfandyari,Ali H. Mokdad,Peter J. Hotez,Robin B. Gasser +8 more
TL;DR: A meta-analysis to estimate the global and regional SARS-CoV-2 seroprevalence rates in humans, and to assess whether serop revalence associates with geographical, climatic and socio-demographic factors, suggested an association with income, human development and climate change.
Journal ArticleDOI
Comparative performance of five commercially available serologic assays to detect antibodies to SARS-CoV-2 and identify individuals with high neutralizing titers.
Eshan U. Patel,Eshan U. Patel,Evan M. Bloch,William Clarke,Yu Hsiang Hsieh,Denali Boon,Yolanda Eby,Reinaldo E Fernandez,Owen R Baker,Morgan Keruly,Charles S. Kirby,Ethan Klock,Kirsten Littlefield,Jernelle Miller,Haley A Schmidt,Philip Sullivan,Estelle Piwowar-Manning,Ruchee Shrestha,Andrew D. Redd,Andrew D. Redd,Richard E. Rothman,David Sullivan,Shmuel Shoham,Arturo Casadevall,Thomas C. Quinn,Thomas C. Quinn,Andrew Pekosz,Aaron A.R. Tobian,Oliver Laeyendecker,Oliver Laeyendecker +29 more
TL;DR: The diagnostic accuracy of five commercially available EIAs (Abbott, Euroimmun, EDI, ImmunoDiagnostics, and Roche) for detection of IgG or total antibodies to SARS-CoV-2 and neutralizing antibodies (nAbs) was evaluated.
Journal ArticleDOI
Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection.
Seyed Hamid Safiabadi Tali,Jason J. LeBlanc,Zubi Sadiq,Oyejide Damilola Oyewunmi,Carolina Camargo,Bahareh Nikpour,Narges Armanfard,Selena M. Sagan,Sana Jahanshahi-Anbuhi +8 more
TL;DR: A review of the technologies used to detect SARS-CoV-2 in clinical laboratories as well as advances made for molecular, antigen-based, and immunological point-of-care testing, including recent developments in sensor and biosensor devices is presented in this paper.
Journal ArticleDOI
Undiagnosed SARS-CoV-2 seropositivity during the first 6 months of the COVID-19 pandemic in the United States.
Heather Kalish,Carleen Klumpp-Thomas,Sally Hunsberger,Holly Ann Baus,Michael P. Fay,Nalyn Siripong,Jing Wang,Jennifer L. Hicks,Jennifer Mehalko,Jameson Travers,Matthew Drew,Kyle Pauly,Jacquelyn Spathies,Tran B. Ngo,Kenneth M. Adusei,Maria Karkanitsa,Jennifer A. Croker,Yan Li,Barry I. Graubard,Lindsay Czajkowski,Olivia Belliveau,Cheryl Chairez,Kelly Snead,Peter Frank,Anandakumar Shunmugavel,Alison Han,Luca T. Giurgea,Luz Angela Rosas,Rachel Bean,Rani Athota,Adriana Cervantes-Medina,Monica Gouzoulis,Brittany Heffelfinger,Shannon Valenti,Rocco Caldararo,Michelle M. Kolberg,Andrew Kelly,Reid Simon,Saifullah Shafiq,Vanessa Wall,Susan Reed,Eric W. Ford,Ravi Lokwani,John-Paul Denson,Simon Messing,Sam Michael,William K. Gillette,Robert P. Kimberly,Steven E. Reis,Matthew D. Hall,Dominic Esposito,Matthew J. Memoli,Kaitlyn Sadtler +52 more
TL;DR: In this paper, the authors analyzed seropositivity in 9089 adults in the United States who had not been diagnosed previously with COVID-19 and found that there were 4.8 undiagnosed SARS-CoV-2 infections for every diagnosed case of COVID19, and an estimated 16.8 million infections were undistributed by mid-July 2020 in the USA.
References
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Journal ArticleDOI
Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019.
Juanjuan Zhao,Quan Yuan,Haiyan Wang,Wei Liu,Xuejiao Liao,Yingying Su,Xin Wang,Jing Yuan,Tingdong Li,Jinxiu Li,Shen Qian,Congming Hong,Fuxiang Wang,Yingxia Liu,Zhaoqin Wang,Qing He,Zhiyong Li,Bin He,Tianying Zhang,Yang Fu,Shengxiang Ge,Lei Liu,Jun Zhang,Ningshao Xia,Zheng Zhang +24 more
TL;DR: The findings provide strong empirical support for the routine application of serological testing in the diagnosis and management of COVID-19 patients and offer vital clinical information during the course of SARS-CoV-2 infection.
Journal ArticleDOI
The diagnostic odds ratio: a single indicator of test performance
TL;DR: The diagnostic odds ratio is closely linked to existing indicators, it facilitates formal meta-analysis of studies on diagnostic test performance, and it is derived from logistic models, which allow for the inclusion of additional variables to correct for heterogeneity.
Journal ArticleDOI
Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2.
Wanbing Liu,Lei Liu,Guomei Kou,Yaqiong Zheng,Yinjuan Ding,Wenxu Ni,Qiongshu Wang,Li Tan,Wanlei Wu,Shi Tang,Zhou Xiong,Shangen Zheng +11 more
TL;DR: ELISA has a high sensitivity, especially for the detection of serum samples from patients after 10 d.p.o., so it could be an important supplementary method for COVID-19 diagnosis.
Journal ArticleDOI
Covid-19: identifying and isolating asymptomatic people helped eliminate virus in Italian village.
TL;DR: How blanket testing in a completely isolated village of roughly 3000 people in northern Italy saw the number of people with covid-19 symptoms fall by over 90% within 10 days is reported.
Journal ArticleDOI
Serology characteristics of SARS-CoV-2 infection after exposure and post-symptom onset.
Bin Lou,Ting Dong Li,Shu Fa Zheng,Yingying Su,Zhiyong Li,Wei Liu,Fei Yu,Shengxiang Ge,Qian Da Zou,Quan Yuan,Sha Lin,Cong Ming Hong,Xiang Yang Yao,Xue Jie Zhang,Ding Hui Wu,Guo Liang Zhou,Wang Heng Hou,Tingting Li,Yali Zhang,Shi Yin Zhang,Jian Fan,Jun Zhang,Ningshao Xia,Yu Chen +23 more
TL;DR: Serology testing provides an important complement to RNA testing in the later stages of illness for pathogenic-specific diagnosis and helpful information to evaluate the adapted immunity status of patients.
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