Book ChapterDOI
Methods for cell and particle tracking.
TLDR
The latest developments in computerized image analysis are surveyed and the various computational approaches, software tools, and quantitative measures for tracking and motion analysis of cells and particles in time-lapse microscopy images are discussed.Abstract:
Achieving complete understanding of any living thing inevitably requires thorough analysis of both its anatomic and dynamic properties. Live-cell imaging experiments carried out to this end often produce massive amounts of time-lapse image data containing far more information than can be digested by a human observer. Computerized image analysis offers the potential to take full advantage of available data in an efficient and reproducible manner. A recurring task in many experiments is the tracking of large numbers of cells or particles and the analysis of their (morpho)dynamic behavior. In the past decade, many methods have been developed for this purpose, and software tools based on these are increasingly becoming available. Here, we survey the latest developments in this area and discuss the various computational approaches, software tools, and quantitative measures for tracking and motion analysis of cells and particles in time-lapse microscopy images.read more
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Journal ArticleDOI
Tumor-Residing Batf3 Dendritic Cells Are Required for Effector T Cell Trafficking and Adoptive T Cell Therapy.
TL;DR: The data indicate that lack of CD103+ DCs within the tumor microenvironment dominantly resists the effector phase of an anti-tumor T cell response, contributing to immune escape.
Journal ArticleDOI
Objective comparison of particle tracking methods
Nicolas Chenouard,Ihor Smal,Fabrice de Chaumont,Martin Maška,Martin Maška,Ivo F. Sbalzarini,Yuanhao Gong,Janick Cardinale,Craig Carthel,Stefano Coraluppi,Mark R. Winter,Andrew R. Cohen,William J. Godinez,Karl Rohr,Yannis Kalaidzidis,Liang Liang,James S. Duncan,Hongying Shen,Yingke Xu,Klas E. G. Magnusson,Joakim Jalden,Helen M. Blau,Perrine Paul-Gilloteaux,Philippe Roudot,Charles Kervrann,François Waharte,Jean-Yves Tinevez,Spencer L. Shorte,Joost Willemse,Katherine Celler,Gilles P. van Wezel,Han-Wei Dan,Yuh-Show Tsai,Carlos Ortiz de Solórzano,Jean-Christophe Olivo-Marin,Erik Meijering +35 more
TL;DR: Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.
Journal ArticleDOI
In the right place at the right time: visualizing and understanding mRNA localization
TL;DR: The emerging movements and localization patterns of mRNAs in morphologically distinct unicellular organisms and in neurons have illuminated shared and specialized mechanisms of mRNA localization, and this information is complemented by transgenic and biochemical techniques that reveal the biological consequences of mRNA mislocalization.
Journal ArticleDOI
Robust Nucleus/Cell Detection and Segmentation in Digital Pathology and Microscopy Images: A Comprehensive Review
Fuyong Xing,Lin Yang +1 more
TL;DR: A comprehensive summary of the recent state-of-the-art nucleus/cell segmentation approaches on different types of microscopy images including bright-field, phase-contrast, differential interference contrast, fluorescence, and electron microscopies is provided.
Journal ArticleDOI
TRAK/Milton motor-adaptor proteins steer mitochondrial trafficking to axons and dendrites.
Myrrhe van Spronsen,Myrrhe van Spronsen,Marina Mikhaylova,Marina Mikhaylova,Joanna Lipka,Joanna Lipka,Max A. Schlager,Dave J. van den Heuvel,Marijn Kuijpers,Phebe S. Wulf,Nanda Keijzer,Jeroen Demmers,Lukas C. Kapitein,Dick Jaarsma,Hans C. Gerritsen,Anna Akhmanova,Casper C. Hoogenraad,Casper C. Hoogenraad +17 more
TL;DR: This study demonstrates how the molecular interplay between bidirectional adaptor proteins and distinct microtubule-based motors drives polarized mitochondrial transport.
References
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Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
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Methods of Digital Video Microscopy for Colloidal Studies
John C. Crocker,David G. Grier +1 more
TL;DR: In this article, a set of image processing algorithms for extracting quantitative data from digitized video microscope images of colloidal suspensions is described, which can locate submicrometer spheres to within 10 nm in the focal plane and 150 nm in depth.
Journal ArticleDOI
SINGLE-PARTICLE TRACKING: Applications to Membrane Dynamics
Michael J. Saxton,Ken Jacobson +1 more
TL;DR: Measurements of trajectories of individual proteins or lipids in the plasma membrane of cells show a variety of types of motion, including directed motion, confined motion, and anomalous diffusion, which requires a revision of existing views of membrane structure and dynamics.
Journal ArticleDOI
Robust single-particle tracking in live-cell time-lapse sequences.
Khuloud Jaqaman,Dinah Loerke,Marcel Mettlen,Hirotaka Kuwata,Sergio Grinstein,Sandra L. Schmid,Gaudenz Danuser +6 more
TL;DR: This approach shows that the GTPase dynamin differentially affects the kinetics of long- and short-lived endocytic structures and that the motion of CD36 receptors along cytoskeleton-mediated linear tracks increases their aggregation probability.
Journal ArticleDOI
Feature point tracking and trajectory analysis for video imaging in cell biology
TL;DR: A computationally efficient, two-dimensional, feature point tracking algorithm for the automated detection and quantitative analysis of particle trajectories as recorded by video imaging in cell biology.