Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
Citations
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Journal ArticleDOI
Correlation of carbonic anhydrase 9 (CA9) with pathological T-stage and prognosis in patients with oral tongue squamous cell carcinoma.
Shuang Wang,Zhiguang Fu,Yizhu Wang,Yaping Sun,Lei Cui,Chunfang Wang,Qiaoling Liu,Dan Shao,Wang Yu,Ning Wen +9 more
TL;DR: Key DEGs were identified that may assist in the molecular understanding of OTSCC and CA9 warrants further exploration as potential prognostic biomarker and therapeutic target in OtsCC.
Journal ArticleDOI
Micro RNA as Biomarkers of Head and Neck Cancers
TL;DR: The aim of this review was to identify microRNA patterns specific for oral squamous cell carcinoma, salivary glands and odontogenic tumors and to correlate a microRNA signature to the diagnosis and prognosis of these tumors.
Journal ArticleDOI
Role of dysregulated miRNAs profiles as hallmarks in the pathogenesis and differential regulation of tongue squamous cell carcinoma.
Journal ArticleDOI
Novel Dysregulated MicroRNAs in Primary Laryngeal Squamous Cell Cancer
Josena K. Stephen,Kang Mei Chen,Veena Shah,Vanessa G. Schweitzer,Glendon M. Gardner,Maria J. Worsham,Shaleta Havard +6 more
TL;DR: 15 novel miRNAs identified in this exploratory study may have clinical utility as LSCC-specific markers, pending further confirmation and validation.
Journal ArticleDOI
MicroRNA Expression Profiling of Cutaneous Squamous Cell Carcinomas Arising in Different Sites.
Alexander N. Rock,Mason D Fisher,Gwenyth Amborski,Dawn C. Allain,Victoria Klee,Sara B. Peters,Stephen Y. Kang,Stephen Y. Kang,Amanda E. Toland,Amanda E. Toland +9 more
TL;DR: Of 5 miRNAs evaluated, only miR-21 showed significantly higher expression in tumors from aggressive sites relative to nonaggressive sites, and lSCC behaves more aggressively when originating from specific anatomical subsites of the head and neck.
References
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