Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
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Epigenetic markers for chemosensitivity and chemoresistance in pancreatic cancer--a review.
TL;DR: The potential of genetic and epigenetic markers in the prediction of chemosensitivity in pancreatic cancer patients and in the monitoring of their response rates to adjuvant therapy are illustrated.
Journal ArticleDOI
MicroRNAs in cancer diagnosis and therapy: from bench to bedside.
Isamu Hoshino,Hisahiro Matsubara +1 more
TL;DR: The most recent findings related to miR mechanisms of action, the modification of miR expression, and their relationship to cancer are reviewed and the potential application of miRs in the clinical setting, such as for biomarkers and therapy is discussed.
Journal ArticleDOI
Salivary MicroRNAs for Early Detection of Head and Neck Squamous Cell Carcinoma: A Case-Control Study in the High Altitude Mestizo Ecuadorian Population.
Carolina Salazar-Ruales,Jessica-Viviana Arguello,Andrés López-Cortés,Alejandro Cabrera-Andrade,Jennyfer M. García-Cárdenas,Patricia Guevara-Ramírez,Patricio Peralta,Paola E. Leone,César Paz-y-Miño +8 more
TL;DR: Findings show that altered expression of miRNAs could be used as biomarkers for HNSCC diagnosis in the high altitude mestizo Ecuadorian population.
Journal ArticleDOI
Effects of miR-155 antisense oligonucleotide on breast carcinoma cell line MDA-MB-157 and implanted tumors
TL;DR: It is revealed that miR-155 ASO can induce cell apoptosis and inhibit cell proliferation in vitro and could significantly repress tumor growth in vivo, presumably by inducing apoptosis via caspase-3 up-regulation.
Journal ArticleDOI
MiR-34b/c-5p and the neurokinin-1 receptor regulate breast cancer cell proliferation and apoptosis.
Lufang Zhang,Lushan Wang,Dong Dong,Zhiyong Wang,Wei Ji,Man Yu,Fei Zhang,Ruifang Niu,Yunli Zhou +8 more
TL;DR: The correlation and clinical significance of miR‐34 and NK1R in breast cancer and Neurokinin‐1 receptor is investigated.
References
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Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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