Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
Citations
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Distinctive pattern of let-7 family microRNAs in aggressive carcinoma of the oral tongue in young patients.
TL;DR: A unique miRNA signature in young patients with aggressive oral cavity cancer in patients <30 years old is demonstrated, associated with a distinctive expression pattern of the let-7 family.
Journal ArticleDOI
The role of microRNA in nutritional control
E. N. M. Nolte-’t Hoen,E. van Rooij,Martin Bushell,C.-Y. Zhang,R. H. Dashwood,W. P. T. James,C. Harris,David Baltimore +7 more
TL;DR: The role of intracellular and extracellular miRNAs in nutritional control of various (patho)physiological processes is discussed.
Journal ArticleDOI
MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1
TL;DR: The miR-33a/let-7e-ST8SIA1 axis could be a therapeutic target for CRC patients and is confirmed to be significantly down-regulated in CRC samples and drug resistant cells.
Journal ArticleDOI
The increase of oncogenic miRNA expression in tongue carcinogenesis of a mouse model
Yu Yu Kao,Hsi Feng Tu,Shou Yen Kao,Shou Yen Kao,Kuo Wei Chang,Kuo Wei Chang,Shu Chun Lin,Shu Chun Lin +7 more
TL;DR: The dysregulation of oncogenic miRNAs in murine tongue tumorigenesis, which simulates human counterparts is shown, and increased multiple miRNas in the biofluids may be valuable non-invasive markers in detecting oral carcinogenesis at an early stage.
Journal ArticleDOI
Re-Expression of AKAP12 Inhibits Progression and Metastasis Potential of Colorectal Carcinoma In Vivo and In Vitro
TL;DR: It is demonstrated that AKAP12 may play an important role in tumor growth suppression and the survival of human colorectal cancer.
References
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Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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