Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
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miR-16-5p and miR-145-5p trigger apoptosis in human gingival epithelial cells by down-regulating BACH2.
TL;DR: MiR-16-5p and miR-145- 5p mediate apoptosis induced by TNF-α in human gingival epithelial cells by targeting BACH2 and can restore the effects of T NF-α on GECs.
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The scope of liquid biopsy in the clinical management of oral cancer
TL;DR: In this article , the authors proposed a liquid biopsy-based approach for head and neck cancer detection, which provides real-time assessment of variations in the molecular profile of the whole tumour and enables serial monitoring of the disease status.
Journal ArticleDOI
MicroRNA-21 over expression in umbilical cord blood hematopoietic stem progenitor cells by leukemia microvesicles.
TL;DR: HSPCs showed a significant increase in both microRNA-21 gene expression and cell count after treating with leukemia microvesicles compared with the control group, suggesting that HSPC proliferation followed by micro RNA- 21 gene over expression can be another evidence of a leukemia-like phenotype induction in a healthy target cell by leukemiamicrovesicles.
Journal ArticleDOI
Combined In Situ Hybridization and Immunohistochemistry on Archival Tissues Reveals Stromal microRNA-204 as Prognostic Biomarker for Oral Squamous Cell Carcinoma.
Saroj Rajthala,Harsh Dongre,Himalaya Parajuli,Anjie Min,Elisabeth Sivy Nginamau,Elisabeth Sivy Nginamau,Arild Kvalheim,Stein Lybak,Dipak Sapkota,Anne Christine Johannessen,Anne Christine Johannessen,Daniela Elena Costea,Daniela Elena Costea +12 more
TL;DR: In this article, a robust method for dual visualization of miR and protein (pancytokeratin) by combining chromogen-based in situ hybridization (ISH) and immunohistochemistry (IHC) was established to investigate stromal expression of miRs204 as a putative prognostic biomarker in oral squamous cell carcinoma (OSCC).
Journal ArticleDOI
The scope of liquid biopsy in the clinical management of oral cancer.
TL;DR: In this article, the authors proposed a liquid biopsy-based approach for head and neck cancer detection, which provides real-time assessment of variations in the molecular profile of the whole tumour and enables serial monitoring of the disease status.
References
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