Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
Citations
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Journal ArticleDOI
Regulation of the tumour suppressor PDCD4 by miR-499 and miR-21 in oropharyngeal cancers
Xiaoying Zhang,Harriet E. Gee,Harriet E. Gee,Barbara Rose,Cheok Soon Lee,Jonathan R. Clark,Jonathan R. Clark,Michael Elliott,Jennifer R. Gamble,Murray J. Cairns,Adrian L. Harris,Samantha Khoury,Nham Tran +12 more
TL;DR: Findings highlight the complex interplay between miRNAs and tumour suppressor gene regulation and suggest that PDCD4 loss may be an important step in tonsillar carcinogenesis.
Journal ArticleDOI
Molecular Mechanisms of Chemoresistance in Oral Cancer.
TL;DR: The current understandings on molecular mechanisms of chemoresistance in oral cancer were reviewed, including drug efflux, apoptosis, DNA damage and repair, epithelial mesenchymal transition, autophagy and miRNA.
Journal ArticleDOI
MicroRNA-21 is a novel promising target in cancer radiation therapy.
Jia Liu,Hongcheng Zhu,Xi Yang,Yangyang Ge,Chi Zhang,Qin Qin,Jing Lu,Liangliang Zhan,Hongyan Cheng,Xinchen Sun +9 more
TL;DR: This review of microRNA-21 focuses on miR-21, which has been identified in human cancer tissues, to suggest reasonable strategies for future research and describe the known functions and possible underlying molecular mechanisms of mi R-21 in radiosensitivity and radioresistance.
Journal ArticleDOI
Expression profile of salivary micro RNA-21 and 31 in oral potentially malignant disorders.
TL;DR: The present study proved that mi RNA-21 is a better diagnostic marker than miRNA-31 for OPMD, and among the different lesions, leucoplakia had significant upregulation ofmiRNA-21 and 31.
Altered levels of miR-21, miR-125b-2*, miR-134, miR-155, miR-184, and miR-205 in oral squamous cell carcinoma and association with clinicopathological characteristics
TL;DR: The results corroborate the previous findings on the overexpression of mir-21 and downregulation of miR-138 in OSCC and Computational predictions suggest thatmiR-125b-2* may have a role in alternative splicing.
References
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Journal ArticleDOI
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Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more
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