Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
Citations
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MicroRNA-21: a novel therapeutic target in human cancer.
Xuan Pan,Zhaoxia Wang,Rui Wang +2 more
TL;DR: The mechanisms underlying miR-21-mediated chemoresistance and the potential use of miR -21 as a novel molecular target for cancer chemotherapy are reviewed.
Journal ArticleDOI
miR-31 Ablates Expression of the HIF Regulatory Factor FIH to Activate the HIF Pathway in Head and Neck Carcinoma
Chung Ji Liu,Meng Miao Tsai,Pei Shih Hung,Shou Yen Kao,Tsung-Yun Liu,Kou-Juey Wu,Shih Hwa Chiou,Shu Chun Lin,Kuo Wei Chang +8 more
TL;DR: The findings suggest that miR-31 contributes to the development of HNSCC by impeding FIH to activate HIF under normoxic conditions.
Journal ArticleDOI
Up-regulation of miR-21 mediates resistance to trastuzumab therapy for breast cancer.
TL;DR: It is demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474, SKBR3, and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody, whereas protein expression of the PTEN gene, a miR -21 target, was reduced.
Journal ArticleDOI
Plasma microRNA 210 levels correlate with sensitivity to trastuzumab and tumor presence in breast cancer patients
Eun-Jung Jung,Eun-Jung Jung,Libero Santarpia,Ju-Yeon Kim,Francisco J. Esteva,Erica Moretti,Aman U. Buzdar,Angelo Di Leo,Xiao Feng Le,Robert C. Bast,Soon Tae Park,Lajos Pusztai,George A. Calin +12 more
TL;DR: The authors of this report hypothesized that altered miR expression levels in plasma are associated with sensitivity to trastuzumab in patients with HER‐2 positive breast cancer.
Journal ArticleDOI
High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients
Boye Schnack Nielsen,Stine Jørgensen,Jacob U. Fog,Rolf Søkilde,Ib Jarle Christensen,Ulla Hansen,Nils Brünner,Adam Baker,Søren Møller,Hans Jørgen Nielsen +9 more
TL;DR: It is concluded that miR-21 is primarily a stromal microRNA, which when measured by image analysis identifies a subgroup of stage II colon cancer patients with short disease-free survival.
References
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