Journal ArticleDOI
MiR-21 Indicates Poor Prognosis in Tongue Squamous Cell Carcinomas as an Apoptosis Inhibitor
Jinsong Li,Hong-Zhang Huang,Lijuan Sun,Mei Yang,Chaobin Pan,Wei-liang Chen,Donghui Wu,Zhaoyu Lin,Chunxian Zeng,Yandan Yao,Peter Zhang,Erwei Song +11 more
Abstract:
Purpose: We aim to examine miR-21 expression in tongue squamous cell carcinomas (TSCC) and correlate it with patient clinical status, and to investigate its contribution to TSCC cell growth, apoptosis, and tumorigenesis. Experimental Design: MicroRNA profiling was done in 10 cases of TSCC with microarray. MiR-21 overexpression was quantitated with quantitative reverse transcription-PCR in 103 patients, and correlated to the pathoclinical status of the patients. Immunohistochemistry was used to examine the expression of TPM1 and PTEN , and terminal deoxynucleotidyl transferase–mediated dUTP labeling to evaluate apoptosis. Moreover, miR-21 antisense oligonucleotide (ASO) was transfected in SCC-15 and CAL27 cell lines, and tumor cell growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, adherent colony formation, and soft agar assay, whereas apoptosis was determined by Annexin V assay, cytochrome c release, and caspase 3 assay. Tumorigenesis was evaluated by xenografting SCC-15 cells in nude mice. Results: MiR-21 is overexpressed in TSCC relative to adjacent normal tissues. The level of miR-21 is reversely correlated with TPM1 and PTEN expression and apoptosis of cancer cells. Multivariate analysis showed that miR-21 expression is an independent prognostic factor indicating poor survival. Inhibiting miR-21 with ASO in TSCC cell lines reduces survival and anchorage-independent growth, and induces apoptosis in TSCC cell lines. Simultaneous silencing of TPM1 with siRNA only partially recapitulates the effect of miR-21 ASO. Furthermore, repeated injection of miR-21 ASO suppresses tumor formation in nude mice by reducing cell proliferation and inducing apoptosis. Conclusions: miR-21 is an independent prognostic indicator for TSCC, and may play a role in TSCC development by inhibiting cancer cell apoptosis partly via TPM1 silencing.read more
Citations
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Journal ArticleDOI
Epigenetic mechanisms in oral carcinogenesis
Jacqueline A. Gasche,Ajay Goel +1 more
TL;DR: A comprehensive review of the impact of epigenetics on oral tumorigenesis with a systematic report on aberrant DNA methylation, histone modifications and miRNA regulation in the pathogenesis of OSCC is provided.
Journal ArticleDOI
Salivary micro RNA as a potential biomarker in oral potentially malignant disorders: A systematic review.
TL;DR: This systematic review explored the potential of expression of salivary miRNA in OPMD and revealed upregulated miRNA 184 with an area under the curve (AUC) of 0.86 and miRNA 21 with an AUC of0.68, which proved that these miRNAs are significant in detecting early malignancy in O PMD and should be further analyzed in various populations.
Journal ArticleDOI
MicroRNA Deregulations in Head and Neck Squamous Cell Carcinomas
TL;DR: Based on current literature, microRNA deregulation plays a major role in head and neck/oral cancer.
Journal ArticleDOI
The Clinical Utility of miR-21 as a Diagnostic and Prognostic Marker for Renal Cell Carcinoma
Hala Faragalla,Youssef M. Youssef,Andreas Scorilas,Bishoy Khalil,Nicole M.A. White,Salvador Mejia-Guerrero,Heba W.Z. Khella,Michael A.S. Jewett,Andrew Evans,Zsuzsanna Lichner,Georg A. Bjarnason,Linda Sugar,Magdy I. Attalah,George M. Yousef,George M. Yousef +14 more
TL;DR: In this article, the expression of miR-21 was significantly upregulated in RCC compared with healthy kidney, with the highest levels of expression in ccRCC and pRCC subtypes.
Journal ArticleDOI
Oral and Oropharyngeal squamous cell carcinoma: prognostic and predictive parameters in the etiopathogenetic route
Iacopo Panarese,Gabriella Aquino,Andrea Ronchi,Francesco Longo,Marco Montella,Immacolata Cozzolino,Giuseppe Roccuzzo,Giuseppe Colella,Michele Caraglia,Renato Franco +9 more
TL;DR: This review aims to discuss some of the most relevant and novel genetic, epigenetic, and histological prognostic biomarkers in oral cancer that may aid in stratifying prognostic subgroups and rationalizing treatment decisions.
References
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