Journal ArticleDOI
Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system and suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.Abstract:
Hydroxyurea (HU) treatment of early first instar larvae in Drosophila was previously shown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system. HU treatment resulted in the loss of antennal lobe local interneurons and of a subset of relay interneurons (RI), elements usually projecting to the calyx and the lateral protocerebrum (LPR). Other RI were resistant to HU and still projected to the LPR. However, they formed no collaterals in the calyx region (which was also ablated), suggesting that their survival does not depend on targets in the calyx. Hence, the ablated interneurons were derived from the LNb, whereas the HU-resistant elements originated from neuroblasts which begin to divide later in larval life. Developmental GAL4 expression patterns suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.read more
Citations
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Protection from premature habituation requires functional mushroom bodies in Drosophila.
Summer F. Acevedo,Emmanuil I. Froudarakis,Alexandros K. Kanellopoulos,Efthimios M. C. Skoulakis +3 more
TL;DR: It is demonstrated that wild-type animals diminish their responses to electric shock stimuli with properties characteristic of short- and long-term habituation and a novel modulatory role of the mushroom bodies is revealed on responses to repetitive stimuli in agreement with and complementary to their established roles in olfactory learning and memory.
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Distinct molecular underpinnings of Drosophila olfactory trace conditioning
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Antagonistic roles of Wnt5 and the Drl receptor in patterning the Drosophila antennal lobe
Ying Yao,Yuping Wu,Chong Yin,Rie Ozawa,Toshiro Aigaki,Rene R. Wouda,Jasprina N. Noordermeer,Lee G. Fradkin,Huey Hing +8 more
TL;DR: The findings establish wnt5 as an anterograde signal that is expressed by olfactory axons and demonstrate a previously unappreciated, yet powerful, role for glia in patterning the Drosophila Olfactory map.
BookDOI
Brain development in drosophila melanogaster
TL;DR: The development of the Drosophila Larval Brain and the structure of the adult Brain Techniques for Visualising Clonally Related Progeny Clonal Unit Architecture in the Adult Brain Formation of the Clonal Units During Development Functional Importance of theClonal Units Conclusion.
Journal ArticleDOI
Drosophila rugose Is a Functional Homolog of Mammalian Neurobeachin and Affects Synaptic Architecture, Brain Morphology, and Associative Learning
Karolien Volders,Sabrina Scholz,Jan R. Slabbaert,Anja C. Nagel,Patrik Verstreken,John W.M. Creemers,Patrick Callaerts,Martin Schwärzel +7 more
TL;DR: It is proposed that the detailed compendium of phenotypes exhibited by the Drosophila rg null mutant provided here will serve as a test bed for dissecting the different functional domains of BEACH (for beige and human Chediak– Higashi syndrome) proteins, such as Rugose, mouse NbeA, or Nbea orthologs in other species, suchAs human.
References
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Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
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Associative odor learning in Drosophila abolished by chemical ablation of mushroom bodies
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TL;DR: The results demonstrate that MBs mediate associative odor learning in flies, and that adult flies developing without MBs are unable to perform in a classical conditioning paradigm that tests associative learning of odor cues and electric shock.
Journal ArticleDOI
Drosophila Mushroom Body Mutants are Deficient in Olfactory Learning
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