Journal ArticleDOI
Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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TLDR
Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system and suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.Abstract:
Hydroxyurea (HU) treatment of early first instar larvae in Drosophila was previously shown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system. HU treatment resulted in the loss of antennal lobe local interneurons and of a subset of relay interneurons (RI), elements usually projecting to the calyx and the lateral protocerebrum (LPR). Other RI were resistant to HU and still projected to the LPR. However, they formed no collaterals in the calyx region (which was also ablated), suggesting that their survival does not depend on targets in the calyx. Hence, the ablated interneurons were derived from the LNb, whereas the HU-resistant elements originated from neuroblasts which begin to divide later in larval life. Developmental GAL4 expression patterns suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.read more
Citations
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Stochastic and Arbitrarily Generated Input Patterns to the Mushroom Bodies Can Serve as Conditioned Stimuli in Drosophila.
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A genetically-encoded fluorescent acetylcholine indicator
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References
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Journal ArticleDOI
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Associative odor learning in Drosophila abolished by chemical ablation of mushroom bodies
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Journal ArticleDOI
Drosophila Mushroom Body Mutants are Deficient in Olfactory Learning
TL;DR: Two Drosophila mutants are described in which the connections between the input to and the output from the mushroom bodies is largely interrupted, and the defect seems not to impair learning of color discrimination tasks or operant learning involving visual cues.
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