Journal ArticleDOI
Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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TLDR
Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system and suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.Abstract:
Hydroxyurea (HU) treatment of early first instar larvae in Drosophila was previously shown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system. HU treatment resulted in the loss of antennal lobe local interneurons and of a subset of relay interneurons (RI), elements usually projecting to the calyx and the lateral protocerebrum (LPR). Other RI were resistant to HU and still projected to the LPR. However, they formed no collaterals in the calyx region (which was also ablated), suggesting that their survival does not depend on targets in the calyx. Hence, the ablated interneurons were derived from the LNb, whereas the HU-resistant elements originated from neuroblasts which begin to divide later in larval life. Developmental GAL4 expression patterns suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.read more
Citations
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A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies
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References
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Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TL;DR: The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.
Journal ArticleDOI
The Organization of the Chemosensory System in Drosophila Melanogaster: A Review
TL;DR: This review surveys the organization of the olfactory and gustatory systems in the imago and in the larva of Drosophila melanogaster, both at the sensory and the central level.
Journal ArticleDOI
Detection in situ of genomic regulatory elements in Drosophila.
TL;DR: The P-lacZ fusion gene is an efficient tool for the recovery of elements that may regulate gene expression in Drosophila and for the generation of a wide variety of cell-type-specific markers.
Journal ArticleDOI
Associative odor learning in Drosophila abolished by chemical ablation of mushroom bodies
JS de Belle,Martin Heisenberg +1 more
TL;DR: The results demonstrate that MBs mediate associative odor learning in flies, and that adult flies developing without MBs are unable to perform in a classical conditioning paradigm that tests associative learning of odor cues and electric shock.
Journal ArticleDOI
Drosophila Mushroom Body Mutants are Deficient in Olfactory Learning
TL;DR: Two Drosophila mutants are described in which the connections between the input to and the output from the mushroom bodies is largely interrupted, and the defect seems not to impair learning of color discrimination tasks or operant learning involving visual cues.
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