Journal ArticleDOI
Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system and suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.Abstract:
Hydroxyurea (HU) treatment of early first instar larvae in Drosophila was previously shown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system. HU treatment resulted in the loss of antennal lobe local interneurons and of a subset of relay interneurons (RI), elements usually projecting to the calyx and the lateral protocerebrum (LPR). Other RI were resistant to HU and still projected to the LPR. However, they formed no collaterals in the calyx region (which was also ablated), suggesting that their survival does not depend on targets in the calyx. Hence, the ablated interneurons were derived from the LNb, whereas the HU-resistant elements originated from neuroblasts which begin to divide later in larval life. Developmental GAL4 expression patterns suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.read more
Citations
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Molecular Architecture of Smell and Taste in Drosophila
TL;DR: A critical review of the recent literature in smell and taste studies in Drosophila is provided to provide broad insights into the problem of sensory coding.
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Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain
TL;DR: A sensitive imaging system in the Drosophila brain that couples two-photon microscopy with the specific expression of the calcium-sensitive fluorescent protein, G-CaMP is developed, demonstrating that the response pattern of a given glomerulus is a function of the specificity of a single odorant receptor.
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Spatial Representation of the Glomerular Map in the Drosophila Protocerebrum
TL;DR: In the fruit fly, Drosophila, olfactory sensory neurons expressing a given receptor project to spatially invariant loci in the antennal lobe to create a topographic map of receptor activation, which is represented in higher sensory centers in the brain by projection neurons that innervate the same glomerulus.
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Transformation of olfactory representations in the Drosophila antennal lobe.
TL;DR: Comparison of odor-evoked activity from afferents and postsynaptic neurons in the same glomerulus revealed that second-order neurons display broader tuning and more complex responses than their primary afferente, implicating lateral interactions within the antennal lobe.
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The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis
Christopher Potter,Bosiljka Tasic,Bosiljka Tasic,Emilie V. Russler,Emilie V. Russler,Liang Liang,Liang Liang,Liqun Luo,Liqun Luo +8 more
TL;DR: The utility of the new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster is demonstrated in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction.
References
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Journal ArticleDOI
Structure and Function of the Deutocerebrum in Insects
TL;DR: This work has shown that both areas of the deutocerebrum receive primary sensory fibers from receptor cells in the antenna and most and possibly all axons of olfactory receptor belong to the antennal lobe.
Journal ArticleDOI
Metamorphosis of the central nervous system of Drosophila
TL;DR: The study of the metamorphosis of the central nervous system of Drosophila focused on the ventral CNS, where large numbers of new, adult-specific neurons are added to this basic complement of persisting larval cells.
Journal ArticleDOI
Neural reorganization during metamorphosis of the corpora pedunculata in Drosophila melanogaster
TL;DR: The mushroom body, a major neuropil area in the insect brain, is extensively reorganized during pupation, and this reorganization is one of the most extensive yet found in insect metamorphosis.
Journal ArticleDOI
The origin of postembryonic neuroblasts in the ventral nerve cord of Drosophila melanogaster.
TL;DR: The development of threefold-labelled single precursor cells from the early gastrula stage to late larval stages is traced and BrdU labelling suggests that the embryonic neuroblast itself rather than one of its progeny resumes proliferation as a postembryonic neuroblast.
Journal ArticleDOI
The cyclic AMP phosphodiesterase encoded by the drosophila dunce gene is concentrated in the mushroom body neuropil
TL;DR: Antibodies to the dnc PDE showed that the most intensely stained regions in the adult brain were the mushroom body neuropil--areas previously implicated in learning and memory.
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