Journal ArticleDOI
Neuroblast ablation in Drosophila P[GAL4] lines reveals origins of olfactory interneurons
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Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system and suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.Abstract:
Hydroxyurea (HU) treatment of early first instar larvae in Drosophila was previously shown to ablate a single dividing lateral neuroblast (LNb) in the brain. Early larval HU application to P[GAL4] strains that label specific neuron types enabled us to identify the origins of the two major classes of interneurons in the olfactory system. HU treatment resulted in the loss of antennal lobe local interneurons and of a subset of relay interneurons (RI), elements usually projecting to the calyx and the lateral protocerebrum (LPR). Other RI were resistant to HU and still projected to the LPR. However, they formed no collaterals in the calyx region (which was also ablated), suggesting that their survival does not depend on targets in the calyx. Hence, the ablated interneurons were derived from the LNb, whereas the HU-resistant elements originated from neuroblasts which begin to divide later in larval life. Developmental GAL4 expression patterns suggested that differentiated RI are present at the larval stage already and may be retained through metamorphosis.read more
Citations
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Molecular Architecture of Smell and Taste in Drosophila
TL;DR: A critical review of the recent literature in smell and taste studies in Drosophila is provided to provide broad insights into the problem of sensory coding.
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Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain
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Spatial Representation of the Glomerular Map in the Drosophila Protocerebrum
TL;DR: In the fruit fly, Drosophila, olfactory sensory neurons expressing a given receptor project to spatially invariant loci in the antennal lobe to create a topographic map of receptor activation, which is represented in higher sensory centers in the brain by projection neurons that innervate the same glomerulus.
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Transformation of olfactory representations in the Drosophila antennal lobe.
TL;DR: Comparison of odor-evoked activity from afferents and postsynaptic neurons in the same glomerulus revealed that second-order neurons display broader tuning and more complex responses than their primary afferente, implicating lateral interactions within the antennal lobe.
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The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis
Christopher Potter,Bosiljka Tasic,Bosiljka Tasic,Emilie V. Russler,Emilie V. Russler,Liang Liang,Liang Liang,Liqun Luo,Liqun Luo +8 more
TL;DR: The utility of the new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster is demonstrated in determining cell division patterns of a neuronal lineage and gene function in cell growth and proliferation, and in dissecting neurons responsible for olfactory attraction.
References
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Journal ArticleDOI
Spatial and temporal patterns of neurogenesis in the central nervous system of Drosophila melanogaster
James W. Truman,Michael Bate +1 more
TL;DR: Neurogenesis in the ventral CNS of Drosophila was studied using staining with toluidine blue and birth dating of cells monitored by incorporation of bromodeoxyuridine into DNA.
Journal ArticleDOI
Learning and memory in the honeybee
Martin Hammer,Randolf Menzel +1 more
TL;DR: Since associative learning, especially of the classical type, is well described at the phenomenological and operational level (Rescorla, 1988), it provides a favorable approach in the search for the neural substrate underlying learning and memory.
Journal ArticleDOI
Proliferation pattern of postembryonic neuroblasts in the brain of Drosophila melanogaster
Kei Ito,Yoshiki Hotta +1 more
TL;DR: The spatio-temporal proliferation pattern of postembryonic neuroblasts in the central brain region of the supra-esophageal ganglion of Drosophila melanogaster was studied by labeling DNA replicating cells with 5-bromo-2'-deoxyuridine (BrdU).
Journal ArticleDOI
Neuronal architecture of the antennal lobe in Drosophila melanogaster.
TL;DR: Computer reconstruction of the antennal lobe of Drosophila melanogaster has revealed a total of 35 glomeruli, of which 30 are located in the periphery of the lobe and 5 in its center, and no obvious sexual dimorphism of the glomerular architecture was observed.
Journal ArticleDOI
Subdivision of the drosophila mushroom bodies by enhancer-trap expression patterns
TL;DR: Rather than being homogenous, the Drosophila mushroom bodies are found to be compound neuropils in which parallel subcomponents exhibit discrete patterns of gene expression, and a model for mushroom body function is proposed in which Parallel channels of information flow, perhaps with different computational properties, subserve different behavioral roles.
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