Journal ArticleDOI
No Higher Infectivity But Immune Escape of SARS-CoV-2 501Y.V2 Variants
Qianqian Li,Nie Jianhui,Jiajing Wu,Li Zhang,Ruxia Ding,Haixin Wang,Yue Zhang,Tao Li,Shuo Liu,Mengyi Zhang,Chenyan Zhao,Huan Liu,Lingling Nie,Haiyang Qin,Meng Wang,Qiong Lu,Xiaoyu Li,Junkai Liu,Haoyu Liang,Yi Shi,Yuelei Shen,Liangzhi Xie,Linqi Zhang,Xiaowang Qu,Xiaowang Qu,Wenbo Xu,Weijin Huang,Youchun Wang +27 more
Reads0
Chats0
TLDR
The data suggest that the enhancement of infectivity in mouse may lead to the spillover of 501Y.V2 to mouse, which may compromise the efficacy of monoclonal antibodies, convalescent sera and vaccines.Abstract:
The 501Y.V2 variants contain multiple mutations in the spike region. We found that they had no significant increased effect on infectivity to human cell lines and cells overexpressing human ACE2, but increased infectivity in cells overexpressing mouse ACE2 based on the pseudotyped viruses. The susceptibility of 501Y.V2 variants to many neutralizing monoclonal antibodies decreased significantly. These variants also had a significant reduced effect on the neutralization ability of convalescent and RBD protein immunized sera. The immune resistances were mainly caused by E484K and N501Y mutations located in receptor binding region. These data suggest that the enhancement of infectivity in mouse may lead to the spillover of 501Y.V2 to mouse. The immune resistance of 501Y.V2 may compromise the efficacy of monoclonal antibodies, convalescent sera and vaccines.
Funding: This work was supported by General Program ofNational Natural Science Foundation of China [grant number 82073621], BILL & MELINDA GATES FOUNDATION [Investment ID INV-006379], National Science and Technology Major Projects of Drug Discovery [grant number 2018ZX09101001] and National Science and Technology Major Projects of Infectious Disease [grant number 2017ZX10304402].
Conflict of Interest: All the authors declare no competing interests.
Ethical Approval: The protocol of the animal study was approved by Ethical review Committee for Animal Welfare of The National Institutes for Food and Drug Control.read more
Citations
More filters
Posted ContentDOI
The B1.351 and P.1 variants extend SARS-CoV-2 host range to mice
Xavier Montagutelli,Matthieu Prot,Laurine Levillayer,Eduard Baquero Salazar,Grégory Jouvion,Grégory Jouvion,Laurine Conquet,Flora Donati,Flora Donati,Mélanie Albert,Mélanie Albert,Fabiana Gámbaro,Fabiana Gámbaro,Sylvie Behillil,Sylvie Behillil,Vincent Enouf,Vincent Enouf,Dominique Rousset,Jean Jaubert,Félix A. Rey,Sylvie van der Werf,Sylvie van der Werf,Etienne Simon-Loriere +22 more
TL;DR: This article showed that SARS-CoV-2 VOCs are able to infect common laboratory mice, replicating to high titers in the lungs, and this host range expansion is explained in part by the acquisition of changes at key positions of the receptor binding domain that enable binding to the mouse angiotensin-converting enzyme 2 (ACE2) cellular receptor.
Journal ArticleDOI
Identification of SARS-CoV-2 inhibitors targeting Mpro and PLpro using in-cell-protease assay
Anoop Narayanan,Manju Narwal,Sydney A. Majowicz,Carmine Varricchio,Shay A Toner,Carlo Ballatore,Andrea Brancale,Katsuhiko S. Murakami,Joyce Jose +8 more
TL;DR: In this paper , an antiviral screening strategy involving a novel in-cell protease assay, antiviral and biochemical activity assessments, as well as structural determinations for rapid identification of protease inhibitors with low cytotoxicity was presented.
Journal ArticleDOI
Immune response in COVID-19: what is next?
Qing Li,Ying Wang,Qiang-zheng Sun,Jasmin Knopf,Martin Herrmann,Liangyu Lin,Jingting Jiang,Changshun Shao,Peishan Li,Xiaozhou He,Fei Hua,Zubiao Niu,Chaobing Ma,Yichao Zhu,Giuseppe Ippolito,Mauro Piacentini,Jérôme Estaquier,Sonia Melino,Felix D. Weiss,Emanuele Andreano,Eicke Latz,Joachim L. Schultze,Rino Rappuoli,Alberto Mantovani,Tak W. Mak,Gerry Melino,Yufang Shi +26 more
TL;DR: In this article , a review of the current literature on the coronavirus disease 2019 (COVID-19) has been a global pandemic for more than 2 years and it still impacts our daily lifestyle and quality in unprecedented ways.
Journal ArticleDOI
Replication kinetics and infectivity of SARS-CoV-2 variants of concern in common cell culture models
TL;DR: In this paper , a systematic comparison of all SARS-CoV-2 Variants of Concern (VOCs) with altered epidemiological, immunological, and pathogenic properties is presented.
Journal ArticleDOI
Omicron SARS-CoV-2 mutations stabilize spike up-RBD conformation and lead to a non-RBM-binding monoclonal antibody escape
Zhennan Zhao,Jingya Zhou,M. Tian,Min Huang,Sheng Liu,Yufeng Xie,Pu Han,Chongzhi Bai,Pengcheng Han,Anqi Zheng,Lutang Fu,Yuanzhu Gao,Qi Peng,Ying Li,Yan Chai,Zengli Zhang,Xin Zhao,Hao Song,Jianxun Qi,Qihui Wang,Peiyi Wang,George F. Gao +21 more
TL;DR: Systematic structural analyses on apo Omicron spike and its complexes with human ACE2 or S309 neutralizing antibody by cryo-EM found that S371L, S373P and S375F substitutions enhance the stability of the one-RBD-up conformation to prevent exposing more up-R BDs triggered by ACE2 binding.
Related Papers (5)
SARS-CoV-2 501Y.V2 variants lack higher infectivity but do have immune escape.
Qianqian Li,Jianhui Nie,Jiajing Wu,Li Zhang,Ruxia Ding,Haixin Wang,Yue Zhang,Tao Li,Shuo Liu,Mengyi Zhang,Chenyan Zhao,Huan Liu,Lingling Nie,Haiyang Qin,Meng Wang,Qiong Lu,Xiaoyu Li,Junkai Liu,Haoyu Liang,Yi Shi,Yuelei Shen,Liangzhi Xie,Linqi Zhang,Xiaowang Qu,Wenbo Xu,Weijin Huang,Youchun Wang +26 more
SARS-CoV-2 variants show resistance to neutralization by many monoclonal and serum-derived polyclonal antibodies
Michael S. Diamond,Rita Chen,Xuping Xie,James Brett Case,Xianwen Zhang,Laura VanBlargan,Yang Liu,Jianying Liu,John M. Errico,Emma S. Winkler,Naveenchandra Suryadevara,Stephen Tahan,Jackson S. Turner,Wooseob Kim,Aaron J. Schmitz,Mahima Thapa,David Wang,Andrianus Boon,Dora Pinto,Rachel M. Presti,Jane A. O’Halloran,Alfred H.J. Kim,Parakkal Deepak,Daved H. Fremont,Davide Corti,Herbert W. Virgin,James E. Crowe,Lindsay Droit,Ali H. Ellebedy,Pei Yong Shi,Pavlo Gilchuk +30 more
Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies.
Rita E. Chen,Xianwen Zhang,James Brett Case,Emma S. Winkler,Yang Liu,Laura A. VanBlargan,Jianying Liu,John M. Errico,Xuping Xie,Naveenchandra Suryadevara,Pavlo Gilchuk,Seth J. Zost,Stephen Tahan,Lindsay Droit,Jackson S. Turner,Wooseob Kim,Aaron J. Schmitz,Mahima Thapa,David Wang,Adrianus C. M. Boon,Rachel M. Presti,Jane A. O’Halloran,Alfred H.J. Kim,Parakkal Deepak,Dora Pinto,Daved H. Fremont,James E. Crowe,Davide Corti,Herbert W. Virgin,Herbert W. Virgin,Ali H. Ellebedy,Pei Yong Shi,Michael S. Diamond +32 more
mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants
Zijun Wang,Fabian Schmidt,Yiska Weisblum,Frauke Muecksch,Christopher O. Barnes,Shlomo Finkin,Dennis Schaefer-Babajew,Melissa Cipolla,Christian Gaebler,Jenna Ariel Lieberman,Thiago Y. Oliveira,Zhi Yang,Morgan E. Abernathy,Kathryn E. Huey-Tubman,Arlene Hurley,Martina Turroja,Kamille A. West,Kristie Gordon,Katrina G. Millard,Victor A. Ramos,Justin Da Silva,Jianliang Xu,Robert A. Colbert,Roshni Patel,Juan Dizon,Cecille Unson-O'Brien,Irina Shimeliovich,Anna Gazumyan,Marina Caskey,Pamela J. Bjorkman,Rafael Casellas,Theodora Hatziioannou,Paul D. Bieniasz,Paul D. Bieniasz,Michel C. Nussenzweig,Michel C. Nussenzweig +35 more