No Identical “Mesenchymal Stem Cells” at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels
Benedetto Sacchetti,Alessia Funari,Cristina Remoli,Giuseppe Giannicola,Gesine Kögler,Stefanie Liedtke,Giulio Cossu,Marta Serafini,Maurilio Sampaolesi,Enrico Tagliafico,Elena Tenedini,Isabella Saggio,Pamela Gehron Robey,Mara Riminucci,Paolo Bianco +14 more
TLDR
It is shown that muscle pericytes, which are not spontaneously osteochondrogenic as previously claimed, may indeed coincide with an ectopic perivascular subset of committed myogenic cells similar to satellite cells, and the data support the view that different mesoderm derivatives include distinct classes of tissue-specific committed progenitors, possibly of different developmental origin.Abstract:
A widely shared view reads that mesenchymal stem/stromal cells (“MSCs”) are ubiquitous in human connective tissues, can be defined by a common in vitro phenotype, share a skeletogenic potential as assessed by in vitro differentiation assays, and coincide with ubiquitous pericytes. Using stringent in vivo differentiation assays and transcriptome analysis, we show that human cell populations from different anatomical sources, regarded as “MSCs” based on these criteria and assumptions, actually differ widely in their transcriptomic signature and in vivo differentiation potential. In contrast, they share the capacity to guide the assembly of functional microvessels in vivo, regardless of their anatomical source, or in situ identity as perivascular or circulating cells. This analysis reveals that muscle pericytes, which are not spontaneously osteochondrogenic as previously claimed, may indeed coincide with an ectopic perivascular subset of committed myogenic cells similar to satellite cells. Cord blood-derived stromal cells, on the other hand, display the unique capacity to form cartilage in vivo spontaneously, in addition to an assayable osteogenic capacity. These data suggest the need to revise current misconceptions on the origin and function of so-called “MSCs,” with important applicative implications. The data also support the view that rather than a uniform class of “MSCs,” different mesoderm derivatives include distinct classes of tissue-specific committed progenitors, possibly of different developmental origin.read more
Citations
More filters
Journal ArticleDOI
Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature.
Sowmya Viswanathan,Yufang Shi,Yufang Shi,Jacques Galipeau,Mauro Krampera,Katarina LeBlanc,Ivan Martin,Jan A. Nolta,Donald G. Phinney,Luc Sensebé +9 more
TL;DR: The ISCT MSC committee continues to support the use of the acronym "MSCs" but recommends this be supplemented by tissue-source origin of the cells, which would highlight tissue-specific properties.
Journal ArticleDOI
Mesenchymal stem cells: Cell therapy and regeneration potential.
Christina Brown,Christina McKee,Shreeya Bakshi,Keegan Walker,Eryk Hakman,Sophia Halassy,David Svinarich,Robert Dodds,Chhabi K. Govind,G. Rasul Chaudhry +9 more
TL;DR: The characteristics of MSCs are discussed and it is suggested that the variations in their distinctive features are dependent on the source and method of isolation as well as epigenetic changes during maintenance and growth.
Journal ArticleDOI
Biological functions of mesenchymal stem cells and clinical implications
Abderrahim Naji,Masamitsu Eitoku,Benoit Favier,Frédéric Deschaseaux,Nathalie Rouas-Freiss,Narufumi Suganuma +5 more
TL;DR: The fundamental and advanced concepts of MSC biological features are outlined and the biological functions of M SCs in their basic and translational aspects in therapy for degenerative and/or inflammatory diseases are underlined.
Journal ArticleDOI
Mesenchymal Stromal/Stem Cells in Regenerative Medicine and Tissue Engineering.
TL;DR: The history of MSC research from the initial discovery of their multipotency to the more recent recognition of their perivascular identity in vivo and their extraordinary capacity for immunomodulation and angiogenic signaling is summarized.
Journal ArticleDOI
Clear up this stem-cell mess.
TL;DR: Confusion about mesenchymal stem cells is making it easier for people to sell unproven treatments, warn Douglas Sipp, Pamela G. Robey and Leigh Turner.
References
More filters
Journal ArticleDOI
Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement
Massimo Dominici,K. Le Blanc,Ingo Mueller,I. Slaper-Cortenbach,Frank C. Marini,Diane S. Krause,Robert J. Deans,Armand Keating,Darwin J. Prockop,Edwin M. Horwitz +9 more
TL;DR: The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSC, believing this minimal set of standard criteria will foster a more uniform characterization of MSC and facilitate the exchange of data among investigators.
Journal ArticleDOI
Mesenchymal stem cells
TL;DR: The study of mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self‐cell repair.
Journal ArticleDOI
A perivascular origin for mesenchymal stem cells in multiple human organs
Mihaela Crisan,Solomon Yap,Louis Casteilla,Louis Casteilla,Chien Wen Chen,Mirko Corselli,Tea Soon Park,Gabriella Andriolo,Bin Sun,Bo Zheng,Li Zhang,Cyrille Norotte,Pang-ning Teng,Jeremy Traas,Rebecca C. Schugar,Bridget M. Deasy,Stephen F. Badylak,Hans-Jörg Bühring,Jean-Paul Giacobino,Lorenza Lazzari,Johnny Huard,Bruno Péault +21 more
TL;DR: Blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.
Related Papers (5)
A perivascular origin for mesenchymal stem cells in multiple human organs
Mihaela Crisan,Solomon Yap,Louis Casteilla,Louis Casteilla,Chien Wen Chen,Mirko Corselli,Tea Soon Park,Gabriella Andriolo,Bin Sun,Bo Zheng,Li Zhang,Cyrille Norotte,Pang-ning Teng,Jeremy Traas,Rebecca C. Schugar,Bridget M. Deasy,Stephen F. Badylak,Hans-Jörg Bühring,Jean-Paul Giacobino,Lorenza Lazzari,Johnny Huard,Bruno Péault +21 more