Positive regulation of migration and invasion by vasodilator-stimulated phosphoprotein via Rac1 pathway in human breast cancer cells
Guoge Han,Biao Fan,Yimin Zhang,Xuan Zhou,Yongping Wang,Huimin Dong,Yun Wei,Shengrong Sun,Mingbo Hu,Jingwei Zhang,Lei Wei +10 more
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TLDR
The data showed that the higher expression level of VASP contributed to a higher invasive migration capacity of human breast cancer cells which was controlled by the Rac1 pathway.Abstract:
This study aimed to investigate the role of the cytoskeleton-associated protein vasodilator-stimulated phosphoprotein (VASP) on the migration and invasion of human breast cancer cells and its relationship to Rac1 which is a member of the Rho family and has been found to be implicated in tumorigenesis, invasion and metastasis. We detected the mRNA and protein expression levels of VASP and Rac1 of the non-invasive breast cancer cell line MCF-7 as well as the invasive cell line MDA-MB-231 by RT-PCR and Western blotting. GST pull-down assay was used to examine the activity of Rac1. Accordingly, the cell invasive migration ability was analyzed in a wound-healing assay (2D) and transwell assays (3D migration and invasion). We then used VASP-siRNA to inhibit the expression of VASP in breast cancer cells in order to study the relationship between the VASP expression level and the invasive migration ability of breast cancer cells. Rac1-siRNA was used to decrease the expression of Rac1, and observe its effect on the VASP expression level together with the migration and invasion ability of MCF-7 and MDA-MB-231 cells. Our results revealed that the invasive breast cancer cell line MDA-MB-231 showed a higher Rac1 activity and VASP expression level compared with the non-invasive MCF-7. Inhibition of Rac1 or VASP by siRNA, respectively, decreased the migration and invasion ability of breast cancer cells and the transfection of Rac1 siRNA-mediated reduction of VASP expression at mRNA and protein levels. Collectively, our data showed that the higher expression level of VASP contributed to a higher invasive migration capacity of human breast cancer cells which was controlled by the Rac1 pathway.read more
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Chlorotoxin targets ERα/VASP signaling pathway to combat breast cancer
Ying Wang,Ying Wang,Kai Li,Song Han,Yihao Tian,Peng-Chao Hu,Xiao-Long Xu,Yanqi He,Wenting Pan,Yang Gao,Zun Zhang,Jingwei Zhang,Lei Wei +12 more
TL;DR: A new mechanism of CTX anti‐ER‐positive breast cancer is revealed and it is found that CTX can target ER overexpressing breast tumor in vivo and vasodilator stimulated phosphoprotein (VASP) is a target gene of ERα signaling pathway, andCTX can inhibit breast cancer cell proliferation, migration, and invasion through ERα/VASp signaling pathway.
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Proteomic identification of neoadjuvant chemotherapy-related proteins in bulky stage IB-IIA squamous cervical cancer.
TL;DR: Proteomics can be used to identify the NAC-related proteins in squamous cervical cancer and the change in proteins may be associated with NAC exposure and response, but insight into their relevance requires further study.
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CREB1/Lin28/miR-638/VASP Interactive Network Drives the Development of Breast Cancer.
Peng-Chao Hu,Kai Li,Yihao Tian,Wenting Pan,Ying Wang,Xiao-Long Xu,Yanqi He,Yang Gao,Lei Wei,Jingwei Zhang +9 more
TL;DR: The mechanism of CREB1/Lin28/miR-638/VASP network promoting the development of breast cancer was explained, which further elucidated the mechanism of VASP as a key oncogene, and also provided a theoretical basis for expanding new approaches to tumor biotherapy.
Journal ArticleDOI
Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration
TL;DR: The results suggest that VASP protein regulates osteosarcoma cell migration and metastasis and Rac1 and VASp interaction may be a potential target for osteo-sarcomA treatment.
Journal ArticleDOI
The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor.
Natalia Girola,Alisson L. Matsuo,Carlos R. Figueiredo,Mariana H. Massaoka,Camyla F. Farias,Denise C. Arruda,Ricardo A. Azevedo,Hugo P. Monteiro,Pedro T. Resende-Lara,Rodrigo L. O. R. Cunha,Luciano Polonelli,Luiz R. Travassos +11 more
TL;DR: The Rb9 peptide, which contains the VHCDR 3 sequence of anti-sodium-dependent phosphate transport protein 2B (NaPi2B) monoclonal antibody RebMab200 and displayed antitumor properties, is shown to induced substrate hyperadherence, inhibition of cell migration and matrix invasion in melanoma and other tumor cell lines.
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