Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
Citations
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Recent developments in diisocyanate asthma
Qing Liu,Adam V. Wisnewski +1 more
TL;DR: The potential long-term adverse effects of diisocyanate exposure in sensitized patients underscore the need for further studies to elucidate the pathogenesis of this disease and identify biomarkers for sensitization and susceptibility.
Journal ArticleDOI
Heat shock protein 70 upregulation is related to HLA-DR expression in bronchial asthma. Effects of inhaled glucocorticoids.
Giuseppina Bertorelli,V. Bocchino,X. Zhuo,Alfredo Chetta,M. Del Donno,Antonio Foresi,R. Testi,Dario Olivieri +7 more
TL;DR: The HLA‐DR and hsp70 expression on BAL cells and bronchial biopsies from asthmatics, as well as the effect of low dose fluticasone propionate treatment are studied.
Journal ArticleDOI
Human lung mast cell IL-5 gene and protein expression: temporal analysis of upregulation following IgE-mediated activation.
Jonathan S. Jaffe,Mark C. Glaum,Donald G. Raible,Thomas J. Post,Edward Dimitry,Dinesh Govindarao,Yihe Wang,Edward S. Schulman +7 more
TL;DR: It is concluded that mast cells are analogous to T cells in the requirement of co-stimuli for the production of IL-5 protein, which is consistent with a primary role for mast cell activation directly leading to late-phase airway eosinophilia.
Journal ArticleDOI
Presence in sputum of functional dust mite-specific IgE antibodies in intrinsic asthma.
TL;DR: It is confirmed that IgE production occurs in intrinsic asthma and show that part of this IgE recognizes Der p antigens, and it is postulate that a second signal that promotes IgE-mediated asthmatic responses through FcεRI is lacking in intrinsic Asthma.
Journal ArticleDOI
Early diagnosis of asthma in young children by using non-invasive biomarkers of airway inflammation and early lung function measurements: study protocol of a case-control study
Kim D. G. van de Kant,Kim D. G. van de Kant,Ester M.M. Klaassen,Ester M.M. Klaassen,Quirijn Jöbsis,Quirijn Jöbsis,Annedien J Nijhuis,Onno C. P. van Schayck,Edward Dompeling,Edward Dompeling +9 more
TL;DR: The primary objective of the ADEM study (Asthma DEtection and Monitoring study), is to develop a non-invasive instrument for an early asthma diagnosis in young children, using exhaled inflammatory markers and early lung function measurements, and to gain insight into the pathophysiology and aetiology of asthma.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).