Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
Citations
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Journal ArticleDOI
Th2 cells as targets for therapeutic intervention in allergic bronchial asthma
TL;DR: Experimental approaches targeted Th 2-type effector cytokines, Th2-cell recruitment and Th2 -cell development using the immunomodulatory potential of tolerance-inducing cytokines such as IL-10 or of cytokines that are able to induce a counterbalancing Th1 immune response.
Journal ArticleDOI
Phosphodiesterase 4B is essential for TH2-cell function and development of airway hyperresponsiveness in allergic asthma
S.-L. Catherine Jin,S.-L. Catherine Jin,Sho Goya,Susumu Nakae,Dan Wang,Matthew D. Bruss,Chiaoyin Hou,Dale T. Umetsu,Marco Conti +8 more
TL;DR: By relieving a cAMP-negative constraint, PDE4B plays an essential role in T(H)2-cell activation and dendritic cell recruitment during airway inflammation.
Journal ArticleDOI
Real-time assessment of inflammation and treatment response in a mouse model of allergic airway inflammation
Virna Cortez-Retamozo,Filip K. Swirski,Peter Waterman,Hushan Yuan,Jose-Luiz Figueiredo,Andita Newton,Rabi Upadhyay,Claudio Vinegoni,Rainer H. Kohler,Joseph Blois,Adam W. Smith,Matthias Nahrendorf,Lee Josephson,Ralph Weissleder,Mikael J. Pittet +14 more
TL;DR: The use of an injectable MMP-targeted optical sensor is described that specifically and quantitatively resolves eosinophil activity in the lungs of mice with experimental allergic airway inflammation and the immunosuppressive effects of the glucocorticoid drug dexamethasone are confirmed.
Journal ArticleDOI
Sputum cytokine mapping reveals an ‘IL‐5, IL‐17A, IL‐25‐high’ pattern associated with poorly controlled asthma
Sven Seys,M. Grabowski,Wim Adriaensen,A. Decraene,Ellen Dilissen,Jeroen Vanoirbeek,L J Dupont,Jan Ceuppens,Dominique Bullens +8 more
TL;DR: Asthma is a heterogeneous disease with various clinical, inflammatory and molecular phenotypes and sputum cytokine mRNA expression patterns in an unselected group of adult asthma patients are studied to characterize the underlying inflammation.
Journal ArticleDOI
Lipid-Droplet Formation Drives Pathogenic Group 2 Innate Lymphoid Cells in Airway Inflammation.
Fotios Karagiannis,Schekufe Kharabi Masouleh,Klaus Wunderling,Jayagopi Surendar,Vanessa Schmitt,Alexander Kazakov,Marcel Michla,Michael Hölzel,Christoph Thiele,Christoph Wilhelm +9 more
TL;DR: It is shown that tissue-resident type 2 ILCs (ILC2s) display a distinct metabolic signature upon chronic activation, and ketogenic diet is identified as a potent intervention strategy to treat airway inflammation.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).