Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
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Journal ArticleDOI
Induction and regulation of the IgE response
David B. Corry,Farrah Kheradmand +1 more
TL;DR: A complete understanding of this topic is lacking, while new data have raised questions regarding the precise role of IgE in atopic disease.
Journal ArticleDOI
Central role of immunoglobulin (Ig) E in the induction of lung eosinophil infiltration and T helper 2 cell cytokine production: inhibition by a non-anaphylactogenic anti-IgE antibody.
TL;DR: It is demonstrated that IgE-dependent mechanisms are important in the induction of a Th2 immune response and the subsequent infiltration of eosinophils into the airways and neutralization of IgE, for example, non- anaphylactogenic anti-IgE mAbs may provide a novel therapeutic approach to the treatment of allergic airway disease.
Journal ArticleDOI
Klf4 Expression in Conventional Dendritic Cells Is Required for T Helper 2 Cell Responses
Roxane Tussiwand,Roxane Tussiwand,Bart Everts,Gary E. Grajales-Reyes,Nicole M. Kretzer,Arifumi Iwata,Juhi Bagaitkar,Xiaodi Wu,Rachel O.L. Wong,David A. Anderson,Theresa L. Murphy,Edward J. Pearce,Kenneth M. Murphy +12 more
TL;DR: Evidence is provided that Kruppel-like factor 4 (Klf4) is required in IRF4-expressing cDCs to promote Th2, but not Th17, cell responses in vivo.
Journal ArticleDOI
Abrogation of Bronchial Eosinophilic Inflammation and Airway Hyperreactivity in Signal Transducers and Activators of Transcription (STAT)6-deficient Mice
Toshihiko Akimoto,Fumio Numata,Misato Tamura,Yoshimi Takata,Noriko Higashida,Tohru Takashi,Kiyoshi Takeda,Shizuo Akira +7 more
TL;DR: In STAT6-deficient C57BL/6 mice treated in the same fashion, there were no eosinophilia in BAL and significantly less peribronchial inflammation than in wild- type mice, and STAT6−/− mice had much less airway reactivity than wild-type mice.
Journal ArticleDOI
Distinct types of lung-disease caused by functional subsets of antiviral t-cells
TL;DR: The in vitro characteristics of these antiviral T cell lines therefore predict the pathological effects in vivo, and different forms of viral bronchiolitis can be caused by functionally distinct types of activated T cell.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).