Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
Citations
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Journal ArticleDOI
Chronic Exposure to Innocuous Antigen in Sensitized Mice Leads to Suppressed Airway Eosinophilia That Is Reversed by Granulocyte Macrophage Colony-Stimulating Factor
Filip K. Swirski,Dusan Sajic,Clinton S. Robbins,Beata U. Gajewska,Manel Jordana,Martin R. Stämpfli +5 more
TL;DR: This study shows that exposure to innocuous Ag alone does not lead to persistent eosinophilic airway inflammation, but rather to abrogated eOSinophilia, which can be reversed by GM-CSF.
Journal ArticleDOI
Innate lymphoid cells and asthma
TL;DR: This work reviews the recent data regarding innate lymphoid cells and their role in asthma and describes newly described cell types that produce a variety of cytokines, including IL-5 and IL-13.
Journal ArticleDOI
Down-regulation of Th2 cell-mediated murine peritoneal eosinophilia by antiallergic agents.
Hidetoshi Yamaya,Yuuji Basaki,Michinori Togawa,Michiyo Kojima,Mamoru Kiniwa,Naosuke Matsuura +5 more
TL;DR: The present results strongly suggest that agents capable of down-regulating Th2 cell cytokine production may attenuate allergic inflammation by impairing the recruitment of eosinophils that is mediated by Th2 cells.
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Molecular basis of allergic diseases.
TL;DR: The identification of key cells and molecules involved in the initiation and maintenance of allergic inflammation is likely to become an important target in the treatment of this common group of illnesses.
Journal ArticleDOI
Type-2 innate lymphoid cells in human allergic disease.
TL;DR: How I LC2 traits, discovered in mouse models, have informed research in humans and how newly identified human ILC2 pathways might provide potential therapeutic benefits in the future are discussed.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).