Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
Citations
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Journal ArticleDOI
A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses
Hidenori Takagi,Takachika Hiroi,Lijun Yang,Yoshifumi Tada,Yoshikazu Yuki,Kaoru Takamura,Ryotaro Ishimitsu,Hideyuki Kawauchi,Hiroshi Kiyono,Fumio Takaiwa +9 more
TL;DR: Transgenic rice plants expressing mouse dominant T cell epitope peptides of Cry j I and Cry j II allergens of Japanese cedar pollen are developed as a fusion protein with the soybean seed storage protein glycinin for the induction of oral tolerance to pollen allergens.
Journal ArticleDOI
Proinflammatory and anti-inflammatory cytokine responses in preterm infants with systemic infections.
TL;DR: The results indicate that the counter-regulatory mechanism between the proinflammatory and anti-inflammatory cytokine pathways is probably operational in preterm infants of early gestation.
Journal ArticleDOI
TH2 cytokine expression in bronchoalveolar lavage fluid T lymphocytes and bronchial submucosa is a feature of asthma and eosinophilic bronchitis
Christopher E. Brightling,F A Symon,S S Birring,Peter Bradding,Ian D. Pavord,Andrew J. Wardlaw +5 more
TL;DR: The concept of asthma as a disease associated with activation of T(H)2 lymphocytes in the airway is supported and evidence that these cytokines play a role in the development of airway inflammation in eosinophilic bronchitis is provided.
Journal ArticleDOI
Increased production of IFN-γ and cysteinyl leukotrienes in virus-induced wheezing
Sandrijn M. van Schaik,Debra A. Tristram,Inderjeet S. Nagpal,Karen M. Hintz,R. Charles Welliver,Robert C. Welliver +5 more
TL;DR: An imbalance favoring overproduction of IFN-γ appears to be associated temporarily with virus-induced wheezing, and a possible mechanism is the enhanced release of LTs from eosinophils or mast cells after sensitization by IFn-γ.
Journal ArticleDOI
Molecular pathology of allergic disease: I: Lower airway disease
TL;DR: The understanding of the pathophysiologic features of allergic upper airway disease improves, as well as the relationship between their development and that of lowerAirway disease, new strategies of diagnosis and treatment will allow for more effective modulation of the allergic process and associated morbidity.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).