Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
Citations
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Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity
Ya-Jen Chang,Hye Young Kim,Lee A. Albacker,Nicole Baumgarth,Andrew Neil James Mckenzie,Dirk E. Smith,Rosemarie H. DeKruyff,Dale T. Umetsu +7 more
TL;DR: It is shown in a mouse model that influenza infection acutely induced airway hyper-reactivity (AHR), a cardinal feature of asthma, independently of T helper type 2 (TH2) cells and adaptive immunity.
Journal ArticleDOI
Airway remodeling in asthma.
TL;DR: The prevalence, morbidity, and mortality of asthma have increased since the 1980s, and is the most frequent reason for preventable childhood hospitalizations in the United States.
Journal ArticleDOI
Interleukin 4, but not interleukin 5 or eosinophils, is required in a murine model of acute airway hyperreactivity.
David B. Corry,Hans G. Folkesson,Martha L. Warnock,David J. Erle,Michael A. Matthay,Jeanine P. Wiener-Kronish,Richard M. Locksley +6 more
TL;DR: These data implicate IL-4 generated during the period of lymphocyte priming with antigen in establishing the cascade of responses required to generate airway hyperractivity to inhaled antigen and suggest a role for IL-5 or eosinophils.
Journal ArticleDOI
Role of chitin and chitinase/chitinase-like proteins in inflammation, tissue remodeling, and injury.
Chun Geun Lee,Carla A. Da Silva,Charles S. Dela Cruz,Farida Ahangari,Bing Ma,Min-Jong Kang,Chuan-Hua He,Seyedtaghi Takyar,Jack A. Elias +8 more
TL;DR: Levels of YKL-40 in the lung and serum are increased in asthma and other inflammatory and remodeling disorders and often correlate with disease severity, indicating understanding of the roles of C/CLPs in inflammation, tissue remodeling, and tissue injury in health and disease.
Journal ArticleDOI
Prostaglandin D2 as a Mediator of Allergic Asthma
Toshiyuki Matsuoka,Masakazu Hirata,Hiroyuki Tanaka,Yoshimasa Takahashi,Takahiko Murata,Kenji Kabashima,Yukihiko Sugimoto,Takuya Kobayashi,Fumitaka Ushikubi,Yoshiya Aze,Naomi Eguchi,Yoshihiro Urade,Nobuaki Yoshida,Kazushi Kimura,Akira Mizoguchi,Yoshihito Honda,Hiroichi Nagai,Shuh Narumiya +17 more
TL;DR: In this article, the role of prostaglandin D2 (PGD2) in allergic asthma was investigated by the generation of mice deficient in the PGD receptor (DP).
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).