Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
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Journal ArticleDOI
Invariant Natural Killer T Cells in Asthma and Chronic Obstructive Pulmonary Disease
Pandurangan Vijayanand,Grégory Seumois,Chris Pickard,Robert M. Powell,Gilbert Angco,David Sammut,Stephan D. Gadola,Peter S. Friedmann,Ratko Djukanovic +8 more
TL;DR: Investigating the frequency of invariant natural killer T cells in the airways of subjects with mild or moderately severe asthma and whether an increased number of these cells is a feature of chronic obstructive pulmonary disease (COPD) found them in low numbers.
Journal ArticleDOI
Genetic mapping of a murine locus controlling development of T helper 1/T helper 2 type responses.
James D. Gorham,Mehmet L. Guler,Robert G. Steen,Aaron J. Mackey,Mark J. Daly,Kathy Frederick,William F. Dietrich,Kenneth M. Murphy +7 more
TL;DR: A locus on murine chromosome 11 is identified that controls the maintenance of IL-12 responsiveness, and therefore the subsequent Th1/Th2 response in mouse and humans, suggesting that genetic control of Th2 differentiation in mouse, and of atopy development in humans, may be expressed through similar mechanisms.
Journal ArticleDOI
Regulation of Peroxisome Proliferator-activated Receptor γ Expression in Human Asthmatic Airways: Relationship with Proliferation, Apoptosis, and Airway Remodeling
Laurent Benayoun,Séverine Létuvé,Anne Druilhe,Jorge Boczkowski,Marie-Christine Dombret,Patricia Mechighel,Jérôme Mégret,Guy Lesèche,Michel Aubier,Marina Pretolani +9 more
TL;DR: PPAR gamma is a new indicator of airway inflammation and remodeling in asthma; it may be involved in extracellular matrix remodeling and submucosal cell proliferation; and is a target for steroid therapy.
Journal ArticleDOI
Requirement for CD8+ T cells in the development of airway hyperresponsiveness in a marine model of airway sensitization.
Eckard Hamelmann,A. Oshiba,Janette Paluh,Kathleen Bradley,Joan Loader,Terry A. Potter,Gary L. Larsen,Erwin W. Gelfand +7 more
TL;DR: A critical role for CD8+ T cells in the production of IL-5 and the development of altered airway responsiveness after antigen sensitization through the airways is indicated.
Journal ArticleDOI
Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids
G. M. Moller,Shelley E. Overbeek,C. G. Van Helden-Meeuwsen,J. M. W. Van Haarst,Errol P. Prens,Paul G.H. Mulder,D. S. Postma,Henk C. Hoogsteden +7 more
TL;DR: There are not many data on the presence of dendritic cells in the airways of patients with atopic asthma and on the effects of corticosteroid‐treatment on such dendrites, but these data are likely to be essential for the initiation of allergic immune responses in the lung.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).