Journal ArticleDOI
Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma
Douglas S. Robinson,Qutayba Hamid,Sun Ying,Anne Tsicopoulos,J. Barkans,Andrew Bentley,Christopher Corrigan,Stephen R. Durham,A. B. Kay +8 more
TLDR
Atopic asthma is associated with activation in the bronchi of the interleukin-3, 4, and 5 and GM-CSF gene cluster, a pattern compatible with predominant activation of the TH2-like T-cell population.Abstract:
Background. In atopic asthma, activated T helper lymphocytes are present in bronchial-biopsy specimens and bronchoalveolar-lavage (BAL) fluid, and their production of cytokines may be important in the pathogenesis of this disorder. Different patterns of cytokine release are characteristic of certain subgroups of T helper cells, termed TH1 and TH2, the former mediating delayed-type hypersensitivity and the latter mediating IgE synthesis and eosinophilia. The pattern of cytokine production in atopic asthma is unknown. Methods. We assessed cells obtained by BAL in subjects with mild atopic asthma and in normal control subjects for the expression of messenger RNA (mRNA) for interleukin-2, 3, 4, and 5, granulocytemacrophage colony-stimulating factor (GM-CSF), and interferon gamma by in situ hybridization with 32P-labeled complementary RNA. Localization of mRNA to BAL T cells was assessed by simultaneous in situ hybridization and immunofluorescence and by in situ hybridization after immunomagnetic enrichment or...read more
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Anti-interleukin-9 antibody treatment inhibits airway inflammation and hyperreactivity in mouse asthma model.
Gang Cheng,Masafumi Arima,Kyoko Honda,Hirokuni Hirata,Fukiko Eda,Nozomi Yoshida,Fumiya Fukushima,Yoshiki Ishii,Takeshi Fukuda +8 more
TL;DR: Treatment with anti-IL-9 antibody significantly prevented airway hyperreactivity in response to methacholine inhalation and suggest that blockade of IL-9 may be a new therapeutic strategy for bronchial asthma.
Journal ArticleDOI
IL-5 Is the Predominant Eosinophil-active Cytokine in the Antigen-induced Pulmonary Late-phase Reaction
Tsukasa Ohnishi,H. Kita,Deborah A. Weiler,Sanjiv Sur,Julie Sedgwick,William J. Calhoun,W.W. Busse,JS Abrams,Gerald J. Gleich +8 more
TL;DR: The findings suggest that the eosinophil survival activity in the late inflammatory lesions following SBP with allergen is mainly associated with IL-5, with small contributions from GM-CSF.
Journal ArticleDOI
Serial analysis of gene expression in human monocyte-derived dendritic cells.
Shin-ichi Hashimoto,Takuji Suzuki,Hong Yan Dong,Shigenori Nagai,Nobuyuki Yamazaki,Kouji Matsushima +5 more
TL;DR: The identification of specific genes expressed in human Mo-derived DCs should provide candidate genes to define subsets of, the function of, and the maturation stage of DCs and possibly also to diagnose diseases in which DCs play a significant role, such as autoimmune diseases and neoplasms.
Journal ArticleDOI
The role of the T cell in asthma.
TL;DR: Therapeutic strategies aimed at T(H)2 cytokines are beginning to bear fruit in patients with asthma, although like many biologic agents, these might need specific targeting at subgroups of patients.
Journal ArticleDOI
Innate and adaptive T cells in asthmatic patients: Relationship to severity and disease mechanisms
Timothy S. C. Hinks,Xiaoying Zhou,Karl J. Staples,Borislav D. Dimitrov,Alexander Manta,Tanya C. Petrossian,Pek Yee Lum,Caroline Smith,Jon Ward,Peter H. Howarth,Andrew F. Walls,Stephan D. Gadola,Stephan D. Gadola,Ratko Djukanovic +13 more
TL;DR: This study provides proof of concept for disease mechanistic networks in asthmatic patients with clusters that could inform the development of new therapies, and identifies 6 novel clusters that are associated with diverse underlying disease mechanisms.
References
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Journal ArticleDOI
TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties.
Tim R. Mosmann,R L Coffman +1 more
TL;DR: Two types of cloned helper T cells are described, defined primarily by differences in the pattern of lymphokines ynthesized, and the different functions of the two types of cells and their lymphokine synthesis are discussed.
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal Article
IL-4 directs the development of Th2-like helper effectors.
TL;DR: The concept that different subsets of helper cells, which correspond roughly to Th1 and Th2 subsets, can develop rapidly in short term culture with respectively low vs high levels of IL-4 support the concept that such distinct phenotypes arise from alternate pathways of differentiation.
Journal ArticleDOI
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI.
Kevin W. Moore,Paulo Vieira,David Fiorentino,Mary L. Trounstine,Tariq A. Khan,Tim R. Mosmann +5 more
TL;DR: The predicted protein sequence shows extensive homology with an uncharacterized open reading frame, BCRFI, in the Epstein-Barr virus genome, suggesting the possibility that this herpes virus exploits the biological activity of a captured cytokine gene to enhance its survival in the host.
Journal ArticleDOI
Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
Marshall Plaut,Jacalyn H. Pierce,Cynthia J. Watson,J. Hanley-Hyde,Richard P. Nordan,William E. Paul +5 more
TL;DR: It is shown that cross-linkage of FcεRI on a series of non-transformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2cells).