Journal ArticleDOI
Quantification of the sixth DNA base hydroxymethylcytosine in the brain.
Martin Münzel,Daniel Globisch,Tobias Brückl,Mirko Wagner,Veronika Welzmiller,Stylianos Michalakis,Markus Müller,Martin Biel,Thomas Carell +8 more
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This article is published in Angewandte Chemie.The article was published on 2010-07-19. It has received 384 citations till now. The article focuses on the topics: 5-Hydroxymethylcytosine & DNA.read more
Citations
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Oncometabolite 2-Hydroxyglutarate Is a Competitive Inhibitor of α-Ketoglutarate-Dependent Dioxygenases
Wei Xu,Hui Yang,Ying Liu,Ying Yang,Ping Wang,Se Hee Kim,Shinsuke Ito,Chen Yang,Pu Wang,Meng Tao Xiao,Li Xia Liu,Wen Qing Jiang,Jing Liu,Jin Ye Zhang,Bin Wang,Stephen V. Frye,Yi Zhang,Yanhui Xu,Qun-Ying Lei,Kun-Liang Guan,Shimin Zhao,Yue Xiong +21 more
TL;DR: 2-HG is a competitive inhibitor of multiple α-KG-dependent dioxygenases, including histone demethylases and the TET family of 5-methlycytosine (5mC) hydroxylases, leading to genome-wide histone and DNA methylation alterations.
Journal ArticleDOI
Hydroxylation of 5-Methylcytosine by TET1 Promotes Active DNA Demethylation in the Adult Brain
TL;DR: This study suggests a TET1-induced oxidation-deamination mechanism for active DNA demethylation in mammals.
Journal ArticleDOI
TET-mediated active DNA demethylation: mechanism, function and beyond
Xiaoji Wu,Yi Zhang +1 more
TL;DR: Recent advances in biochemical and structural studies have revealed mechanistic insights into how TET and TDG mediate active DNA demethylation and many regulatory mechanisms of this process have been identified.
Journal ArticleDOI
Base-Resolution Analysis of 5-Hydroxymethylcytosine in the Mammalian Genome
Miao Yu,Gary C. Hon,Keith E. Szulwach,Chun-Xiao Song,Liang Zhang,Audrey Kim,Xuekun Li,Qing Dai,Yin Shen,Beomseok Park,Jung Hyun Min,Peng Jin,Bing Ren,Chuan He +13 more
TL;DR: Application of Tet-assisted bisulfite sequencing to embryonic stem cells not only confirms widespread distribution of 5hmC in the mammalian genome but also reveals sequence bias and strand asymmetry at5hmC sites.
Journal ArticleDOI
Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine
Chun-Xiao Song,Keith E. Szulwach,Ye Fu,Qing Dai,Chengqi Yi,Xuekun Li,Yujing Li,Chih Hsin Chen,Wen Zhang,Xing Jian,Jing Wang,Li Zhang,Timothy J. Looney,Baichen Zhang,Lucy A. Godley,Leslie M. Hicks,Bruce T. Lahn,Peng Jin,Chuan He +18 more
TL;DR: This method uses the T4 bacteriophage β-glucosyltransferase to transfer an engineered glucose moiety containing an azide group onto the hydroxyl group of 5-hmC, a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types.
References
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Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid
James D. Watson,Francis Crick +1 more
TL;DR: The determination in 1953 of the structure of deoxyribonucleic acid (DNA), with its two entwined helices and paired organic bases, was a tour de force in X-ray crystallography and opened the way for a deeper understanding of perhaps the most important biological process.
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Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1
Mamta Tahiliani,Kian Peng Koh,Yinghua Shen,William A. Pastor,Hozefa S. Bandukwala,Yevgeny Brudno,Suneet Agarwal,Lakshminarayan M. Iyer,David R. Liu,L. Aravind,Anjana Rao +10 more
TL;DR: It is shown here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro.
Journal ArticleDOI
Epigenetics in Cancer
TL;DR: This account of epigenetics in cancer reviews the mechanisms and consequences of epigenetic changes in cancer cells and concludes with the implications of these changes for the diagnosis, prognosis, and treatment of cancer.
Journal ArticleDOI
Perceptions of epigenetics
TL;DR: During the past year, more than 2,500 articles, numerous scientific meetings and a new journal were devoted to the subject of epigenetics, portrayed by the popular press as a revolutionary new science — an antidote to the idea that the authors are hard-wired by their genes.
Journal ArticleDOI
The Nuclear DNA Base 5-Hydroxymethylcytosine Is Present in Purkinje Neurons and the Brain
TL;DR: It is shown that, as well as 5mC in mammalian genomes, there are also significant amounts of 5-hydroxymethylcytosine (5hmC) in DNA of Purkinje neurons, which have large nuclei with apparently very little heterochromatin.