Recovery of nearly 8,000 metagenome-assembled genomes substantially expands the tree of life
Donovan H. Parks,Christian Rinke,Maria Chuvochina,Pierre-Alain Chaumeil,Ben J. Woodcroft,Paul N. Evans,Philip Hugenholtz,Gene W. Tyson +7 more
TLDR
The recovery of 7,903 bacterial and archaeal metagenome-assembled genomes increases the phylogenetic diversity represented by public genome repositories and provides the first representatives from 20 candidate phyla.Abstract:
Challenges in cultivating microorganisms have limited the phylogenetic diversity of currently available microbial genomes. This is being addressed by advances in sequencing throughput and computational techniques that allow for the cultivation-independent recovery of genomes from metagenomes. Here, we report the reconstruction of 7,903 bacterial and archaeal genomes from >1,500 public metagenomes. All genomes are estimated to be ≥50% complete and nearly half are ≥90% complete with ≤5% contamination. These genomes increase the phylogenetic diversity of bacterial and archaeal genome trees by >30% and provide the first representatives of 17 bacterial and three archaeal candidate phyla. We also recovered 245 genomes from the Patescibacteria superphylum (also known as the Candidate Phyla Radiation) and find that the relative diversity of this group varies substantially with different protein marker sets. The scale and quality of this data set demonstrate that recovering genomes from metagenomes provides an expedient path forward to exploring microbial dark matter.read more
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Correcting for 16S rRNA gene copy numbers in microbiome surveys remains an unsolved problem.
TL;DR: It is found that regardless of the phylogenetic method tested, 16S GCNs could only be accurately predicted for a limited fraction of taxa, namely taxa with closely to moderately related representatives (≲15% divergence in the 16S rRNA gene) and all considered tools exhibit low predictive accuracy when evaluated against completely sequenced genomes.
Journal ArticleDOI
Major New Microbial Groups Expand Diversity and Alter our Understanding of the Tree of Life
TL;DR: How new genomes have changed the structure of the tree of life and altered the understanding of biology, evolution, and metabolic roles in biogeochemical processes is illustrated.
Posted ContentDOI
Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3
Francesco Beghini,Lauren J. McIver,Aitor Blanco-Míguez,Leonard Dubois,Francesco Asnicar,Sagun Maharjan,Sagun Maharjan,Ana Mailyan,Ana Mailyan,Andrew Maltez Thomas,Paolo Manghi,Mireia Valles-Colomer,George Weingart,George Weingart,Yancong Zhang,Yancong Zhang,Moreno Zolfo,Curtis Huttenhower,Curtis Huttenhower,Eric A. Franzosa,Eric A. Franzosa,Nicola Segata,Nicola Segata +22 more
TL;DR: With open-source implementations and cloud-deployable reproducible workflows, the bioBakery 3 platform can help researchers deepen the resolution, scale, and accuracy of multi-omic profiling for microbial community studies.
Journal ArticleDOI
A genomic catalog of Earth’s microbiomes
Stephen Nayfach,Simon Roux,Rekha Seshadri,Daniel W. Udwary,Neha Varghese,Frederik Schulz,Dongying Wu,David Paez-Espino,I-Min Chen,Marcel Huntemann,Krishna Palaniappan,Joshua Ladau,Supratim Mukherjee,T. B. K. Reddy,Torben Nielsen,Edward Kirton,José P. Faria,Janaka N. Edirisinghe,Christopher S. Henry,Sean P. Jungbluth,Dylan Chivian,Paramvir S. Dehal,Elisha M. Wood-Charlson,Adam P. Arkin,Susannah G. Tringe,Axel Visel,Tanja Woyke,Nigel J Mouncey,Natalia Ivanova,Nikos C. Kyrpides,Emiley A. Eloe-Fadrosh +30 more
TL;DR: The utility of this collection of >10,000 metagenomes collected from diverse habitats covering all of Earth’s continents and oceans is demonstrated for understanding secondary-metabolite biosynthetic potential and for resolving thousands of new host linkages to uncultivated viruses.
Journal Article
Genome Project Standards in a New Era of Sequencing
TL;DR: There is an urgent need to distinguish good from poor data sets in genome sequences, as there is an ever-widening gap between drafted and finished genomes that only promises to continue.
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