Journal ArticleDOI
Routine In Vitro Culture of Plasmodium falciparum: Experimental Consequences?
Sandra Duffy,Vicky M. Avery +1 more
TLDR
It is reasoned that culture conditions should be re-established as a primary consideration in in vitro malaria experimentation.About:
This article is published in Trends in Parasitology.The article was published on 2018-07-01. It has received 23 citations till now.read more
Citations
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Journal ArticleDOI
Inhibition of Plasmepsin V Activity Blocks Plasmodium falciparum Gametocytogenesis and Transmission to Mosquitoes
Charlie Jennison,Leonardo Lucantoni,Matthew T. O'Neill,Robyn McConville,Robyn McConville,Sara M. Erickson,Sara M. Erickson,Alan F. Cowman,Alan F. Cowman,Brad E. Sleebs,Brad E. Sleebs,Vicky M. Avery,Justin A Boddey,Justin A Boddey +13 more
TL;DR: It is shown that PMV is expressed and can be inhibited specifically in P. falciparum stage I-II gametocytes and primes gametocyte effectors for export, which is essential for the development and fitness ofgametocytes for transmission to mosquitoes.
Journal ArticleDOI
Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies
Regina Hoo,Ellen Bruske,Sandra Dimonte,Lei Zhu,Benjamin Mordmüller,B. Kim Lee Sim,Peter G. Kremsner,Stephen L. Hoffman,Zbynek Bozdech,Matthias Frank,Peter R. Preiser +10 more
TL;DR: Significant transcriptome heterogeneity exits between parasites derived from human infections and the pre-mosquito strain, implying that the malaria parasites undergo a change in functional state to adapt to its host environment.
Journal ArticleDOI
Current status and challenges in drug discovery against the globally important zoonotic cryptosporidiosis
TL;DR: For developing both systemic and non-systemic drugs, a non-ruminant animal model exhibiting diarrheal symptoms suitable for routine evaluation of drug absorption and anti-cryptosporidial efficacy may be very helpful.
Journal ArticleDOI
Cryogenically preserved RBCs support gametocytogenesis of Plasmodium falciparum in vitro and gametogenesis in mosquitoes.
TL;DR: Basic guidelines are presented for integrating cryo-preserved RBCs into an existing laboratory/insectary framework for P. falciparum SMFAs with significant potential for reducing running costs while achieving greater reliability.
References
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Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
Journal ArticleDOI
Genome sequence of the human malaria parasite Plasmodium falciparum
Malcolm J. Gardner,Neil Hall,Eula Fung,Owen White,Matthew Berriman,Richard W. Hyman,Jane M. Carlton,Arnab Pain,Karen E. Nelson,Sharen Bowman,Ian T. Paulsen,Keith D. James,Jonathan A. Eisen,Kim Rutherford,Steven L. Salzberg,Alister Craig,Sue Kyes,Man Suen Chan,Vishvanath Nene,Shamira J. Shallom,Bernard B. Suh,Jeremy Peterson,Samuel V. Angiuoli,Mihaela Pertea,Jonathan E. Allen,Jeremy D. Selengut,Daniel H. Haft,Michael W. Mather,Akhil B. Vaidya,David M. A. Martin,Alan H. Fairlamb,Martin Fraunholz,David S. Roos,Stuart A. Ralph,Geoffrey I. McFadden,Leda M. Cummings,G. Mani Subramanian,Christopher J. Mungall,J. Craig Venter,Daniel J. Carucci,Stephen L. Hoffman,Chris I. Newbold,Ronald W. Davis,Claire M. Fraser,Bart Barrell +44 more
TL;DR: The genome sequence of P. falciparum clone 3D7 is reported, which is the most (A + T)-rich genome sequenced to date and is being exploited in the search for new drugs and vaccines to fight malaria.
Journal ArticleDOI
Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique.
TL;DR: A rapid, semiautomated microdilution method was developed for measuring the activity of potential antimalarial drugs against cultured intraerythrocytic asexual forms of the human malaria parasite Plasmodium falciparum, and results demonstrated that the method is sensitive and precise.
Journal ArticleDOI
A molecular marker of artemisinin-resistant Plasmodium falciparum malaria
Frédéric Ariey,Benoit Witkowski,Chanaki Amaratunga,Johann Beghain,Anne-Claire Langlois,Nimol Khim,Saorin Kim,Valentine Duru,Christiane Bouchier,Laurence Ma,Pharath Lim,Rithea Leang,Socheat Duong,Sokunthea Sreng,Seila Suon,Char Meng Chuor,Denis Mey Bout,Sandie Menard,William O. Rogers,Blaise Genton,Thierry Fandeur,Olivo Miotto,Pascal Ringwald,Jacques Le Bras,Antoine Berry,Jean Christophe Barale,Rick M. Fairhurst,Françoise Benoit-Vical,Odile Mercereau-Puijalon,Didier Menard +29 more
TL;DR: Strong correlations between the presence of a mutant allele, in vitro parasite survival rates and in vivo parasite clearance rates indicate that K13-propeller mutations are important determinants of artemisinin resistance.
Journal ArticleDOI
Thousands of chemical starting points for antimalarial lead identification
Francisco-Javier Gamo,Laura M. Sanz,J. Vidal,Cristina de Cozar,Emilio Alvarez,J.L. Lavandera,Dana E. Vanderwall,Darren V. S. Green,Vinod Kumar,Samiul Hasan,James R. Brown,Catherine E. Peishoff,Lon R. Cardon,Jose F. Garcia-Bustos +13 more
TL;DR: Chemical structures and associated data suggest several novel mechanisms of antimalarial action, such as inhibition of protein kinases and host–pathogen interaction related targets.
Related Papers (5)
Plasmodium falciparum in vitro continuous culture conditions: A comparison of parasite susceptibility and tolerance to anti-malarial drugs throughout the asexual intra-erythrocytic life cycle.
Sandra Duffy,Vicky M. Avery +1 more