Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase.
TLDR
A regulatory network, the FEAR (Cdc fourteen early anaphase release) network that promotes Cdc14 release from the nucleolus during earlyAnaphase is identified and it is proposed that one function of CDC14 released by the Fear network is to stimulate MEN activity.About:
This article is published in Cell.The article was published on 2002-01-25 and is currently open access. It has received 476 citations till now. The article focuses on the topics: Anaphase & Separase.read more
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The anaphase-promoting complex: proteolysis in mitosis and beyond.
TL;DR: Key events in mitosis such as sister chromatid separation and subsequent inactivation of cyclin-dependent kinase 1 are regulated by ubiquitin- dependent proteolysis, mediated by the anaphase-promoting complex.
Journal ArticleDOI
Integrative Analysis of Cell Cycle Control in Budding Yeast
Katherine C. Chen,Laurence Calzone,Attila Csikász-Nagy,Frederick R. Cross,Bela Novak,John J. Tyson +5 more
TL;DR: It is shown that a mathematical model built on a consensus picture of this control system is largely successful in explaining the phenotypes of mutants described so far and allows one to frame and critique hypotheses about how the division cycle is regulated in wild-type and mutant cells, to predict the phenotypic properties of new mutant combinations, and to estimate the effective values of biochemical rate constants that are difficult to measure directly in vivo.
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Multiple levels of cyclin specificity in cell-cycle control.
Joanna Bloom,Frederick R. Cross +1 more
TL;DR: In budding yeast, a single Cdk is activated by multiple cyclins, and the ability of these cyclins to target specific proteins and to initiate different cell-cycle events might reflect the timing of the expression of the cyclins.
Journal ArticleDOI
The anaphase-promoting complex: it's not just for mitosis any more
TL;DR: Recent efforts to understand how central components of this regulatory apparatus—cyclin-dependent kinases and the anaphasepromoting complex/cyclosome (APC/C)—control progression through the cell division cycle and how regulatory mechanisms impinge on the APC/ C are described.
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Polo on the Rise-from Mitotic Entry to Cytokinesis with Plk1.
TL;DR: This review focuses on how Plk1 is recruited to centrosomes, kinetochores, and the spindle midzone and what the specific tasks of PlK1 at these distinct subcellular structures might be.
References
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Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
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Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
TL;DR: It is shown that Esp1 causes the dissociation of Scc1 from chromosomes by stimulating its cleavage by proteolysis, and a mutant SCC1 is described that is resistant to Esp1-dependent cleavage and which blocks both sister-chromatid separation and the dissociations from chromosomes.
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Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.
TL;DR: It is shown that in yeast cells and in mitotic Xenopus extracts Pds1p is degraded in an APC-dependent manner and is directly ubiquitinated by the Xenopus APC.
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The Phosphatase Cdc14 Triggers Mitotic Exit by Reversal of Cdk-Dependent Phosphorylation
TL;DR: This work shows that the Cdc14 phosphatase triggers mitotic exit by three parallel mechanisms, each of which inhibits Cdk activity, and induces degradation of mitotic cyclins.
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Exit from Mitosis Is Triggered by Tem1-Dependent Release of the Protein Phosphatase Cdc14 from Nucleolar RENT Complex
Wenying Shou,Jae Hong Seol,Anna Shevchenko,Christopher Baskerville,Danesh Moazed,Z.W.Susan Chen,Joanne W. Jang,Andrej Shevchenko,Harry Charbonneau,Raymond J. Deshaies +9 more
TL;DR: A mutation is identified, net1-1, that bypasses the lethality of tem1 delta and is a key component of a multifunctional complex, denoted RENT (for regulator of nucleolar silencing and telophase), that also contains Cdc14 and the silencing regulator Sir2.