Journal ArticleDOI
The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a
Sonia Guil,Javier F. Cáceres +1 more
TLDR
It is shown that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing, which underscores a previously uncharacterized role for general RNA-binding proteins as auxiliary factors that facilitate the processing of specific miRNAs.Abstract:
hnRNP A1 is an RNA-binding protein involved in various aspects of RNA processing. Use of an in vivo cross-linking and immunoprecipitation protocol to find hnRNP A1 RNA targets resulted in the identification of a microRNA (miRNA) precursor, pre-miR-18a. This microRNA is expressed as part of a cluster of intronic RNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92, and potentially acts as an oncogene. Here we show that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing. HeLa cells depleted of hnRNP A1 have reduced in vitro processing activity with pri-miR-18a and also show reduced abundances of endogenous pre-miR-18a. Furthermore, we show that hnRNP A1 is required for miR-18a–mediated repression of a target reporter in vivo. These results underscore a previously uncharacterized role for general RNA-binding proteins as auxiliary factors that facilitate the processing of specific miRNAs.read more
Citations
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Regulation of microRNA biogenesis
Minju Ha,V. Narry Kim +1 more
TL;DR: Small non-coding RNAs that function as guide molecules in RNA silencing are involved in nearly all developmental and pathological processes in animals and their dysregulation is associated with many human diseases.
Journal ArticleDOI
Biogenesis of small RNAs in animals.
TL;DR: This Review summarizes the current knowledge of how these intriguing molecules are generated in animal cells.
Journal ArticleDOI
Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIP
Markus Hafner,Markus Landthaler,Lukas Burger,Mohsen Khorshid,Jean Hausser,Philipp Berninger,Andrea Rothballer,Manuel Ascano,Anna-Carina Jungkamp,Mathias Munschauer,Alexander Ulrich,Greg S. Wardle,Scott Dewell,Mihaela Zavolan,Thomas Tuschl +14 more
TL;DR: This study developed a cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs and revealed that these factors bind thousands of sites containing defined sequence motifs and have distinct preferences for exonic versus intronic or coding versus untranslated transcript regions.
Journal ArticleDOI
Many roads to maturity: microRNA biogenesis pathways and their regulation
TL;DR: Recent advances in knowledge of the microRNA biosynthesis pathways are reviewed and their impact on post-transcriptional microRNA regulation during tumour development is discussed.
Journal ArticleDOI
MicroRNA biogenesis pathways in cancer
Shuibin Lin,Richard I. Gregory +1 more
TL;DR: Global miRNA depletion caused by genetic and epigenetic alterations in components of the miRNA biogenesis machinery is oncogenic, highlighting the importance of miRNA dysregulation in cancer.
References
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TL;DR: It is shown that many 21- and 22-nt expressed RNAs, termed microRNAs, exist in invertebrates and vertebrates and that some of these novel RNAs are highly conserved, which suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by smallRNAs are more general than previously appreciated.
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Journal ArticleDOI
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