Institution
University of Córdoba (Spain)
Education•Cordova, Spain•
About: University of Córdoba (Spain) is a education organization based out in Cordova, Spain. It is known for research contribution in the topics: Population & Catalysis. The organization has 12006 authors who have published 22998 publications receiving 537842 citations. The organization is also known as: University of Córdoba (Spain) & Universidad de Córdoba.
Papers published on a yearly basis
Papers
More filters
••
TL;DR: The need for the introduction of molecular techniques such as chromosome painting and physical mapping of genetic markers for the identification of small acrocentrics is discussed.
Abstract: Karyotyping of dog chromosomes is a difficult task owing to the high diploid number of chromosomes (2n=78) and the similar morphology of autosomes, all of which are acrocentrics. In this report 22 of the 39 G-banded chromosome pairs and their corresponding ideograms have been standardized. The ideogram comprises altogether 235 bands. The need for the introduction of molecular techniques such as chromosome painting and physical mapping of genetic markers for the identification of small acrocentrics is discussed.
113 citations
••
TL;DR: A metallomic analytical approach (MAA) is proposed as a new tool to study and identify metalloproteins in proteomics, connected with the presence of metals bound to biomolecules, which adds an additional dimension to metal‐biomolecule and meetalloprotein characterization.
Abstract: Monitoring environmental pollution using biomarkers requires detailed knowledge about the markers, and many only allow a partial assessment of pollution. New proteomic methods (environmental proteomics) can identify proteins that, after validation, might be useful as alternative biomarkers, although this approach also has its limitations, derived mainly from their application to non-model organisms. Initial studies using environmental proteomics were carried out in animals exposed to model pollutants, and led to the concept of protein expression signatures. Experiments have been carried out in model organisms (yeast, Arabidopsis, rat cells, or mice) exposed to model contaminants. Over the last few years, proteomics has been applied to organisms from ecosystems with different pollution levels, forming the basis of an environmental branch in proteomics. Another focus is connected with the presence of metals bound to biomolecules, which adds an additional dimension to metal-biomolecule and metalloprotein characterization - the field of metallomics. The metallomic approach considers the metallome: a whole individual metal or metalloid species within a cell or tissue. A metallomic analytical approach (MAA) is proposed as a new tool to study and identify metalloproteins.
113 citations
••
TL;DR: The proposed approach - which provides more concentrated extracts than previous alternatives - is very useful to study matrix-extractant analytes partition and its easy automation and low acquisition and maintenance costs make the industrial implementation of the proposed method advisable.
112 citations
••
TL;DR: The low variation in the cultivated olive tree indicated that the utility of cpDNA variation for forensic analyses is limited to rare haplotypes, and the high cp DNA variation in wild populations demonstrated that the markers may be useful for phylogeographic and populations genetic studies in O. europaea.
Abstract: Characterisation of plastid genome (or cpDNA) polymorphisms is commonly used for phylogeographic, population genetic and forensic analyses in plants, but detecting cpDNA variation is sometimes challenging, limiting the applications of such an approach. In the present study, we screened cpDNA polymorphism in the olive tree (Olea europaea L.) by sequencing the complete plastid genome of trees with a distinct cpDNA lineage. Our objective was to develop new markers for a rapid genomic profiling (by Multiplex PCRs) of cpDNA haplotypes in the Mediterranean olive tree. Eight complete cpDNA genomes of Olea were sequenced de novo. The nucleotide divergence between olive cpDNA lineages was low and not exceeding 0.07%. Based on these sequences, markers were developed for studying two single nucleotide substitutions and length polymorphism of 62 regions (with variable microsatellite motifs or other indels). They were then used to genotype the cpDNA variation in cultivated and wild Mediterranean olive trees (315 individuals). Forty polymorphic loci were detected on this sample, allowing the distinction of 22 haplotypes belonging to the three Mediterranean cpDNA lineages known as E1, E2 and E3. The discriminating power of cpDNA variation was particularly low for the cultivated olive tree with one predominating haplotype, but more diversity was detected in wild populations. We propose a method for a rapid characterisation of the Mediterranean olive germplasm. The low variation in the cultivated olive tree indicated that the utility of cpDNA variation for forensic analyses is limited to rare haplotypes. In contrast, the high cpDNA variation in wild populations demonstrated that our markers may be useful for phylogeographic and populations genetic studies in O. europaea.
112 citations
••
TL;DR: This work examines domestication from a population genetics perspective, with a focus on three issues: genetic bottlenecks during domestication, introgression as a source of local adaptation, and genetic load.
112 citations
Authors
Showing all 12089 results
Name | H-index | Papers | Citations |
---|---|---|---|
Jose M. Ordovas | 123 | 1024 | 70978 |
Liang Cheng | 116 | 1779 | 65520 |
Pedro W. Crous | 115 | 809 | 51925 |
Munther A. Khamashta | 109 | 623 | 50205 |
Luis Serrano | 105 | 452 | 42515 |
Raymond Vanholder | 103 | 841 | 40861 |
Carlos Dieguez | 101 | 545 | 36404 |
David G. Bostwick | 99 | 403 | 31638 |
Leon V. Kochian | 95 | 266 | 31301 |
Abhay Ashtekar | 94 | 366 | 37508 |
Néstor Armesto | 93 | 369 | 26848 |
Manuel Hidalgo | 92 | 538 | 41330 |
Rafael de Cabo | 91 | 317 | 35020 |
Harald Mischak | 90 | 445 | 27472 |
Manuel Tena-Sempere | 87 | 351 | 23100 |