A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
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Dissertation
Molecular Mechanisms of Protein Kinase A Signaling Pathway on Estrogen Receptor Action in Breast Cancer
Dissertation
Cell cycle deregulation in breast cancer subgroups and effects on proliferation, migration and tamoxifen resistance
TL;DR: It is shown that the consequences of targeting cell cycle proteins may differ depending on ER expression, and a non-functional pathway of the cell-cycle regulator RB and absent YAP1 protein expression to impaired tamoxifen response is linked, identifying two potential biomarkers for predicting tamoxIFen insensitivity.
Book ChapterDOI
Chapter 9 Molecular aspects of steroid receptor/DNA binding
TL;DR: The general principles used by transcription factors, and specifically steroid receptors, to accomplish the needed specificity and affinity for DNA is described in this chapter.
References
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Book
Gene Regulation by Steroid Hormones II
Arun K. Roy,James H. Clark +1 more
TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
Book
Molecular biology of egg maturation
Ruth Porter,Julie Whelan +1 more
TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?