A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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TLDR
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
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Journal ArticleDOI
Definition of the DNA-binding site repertoire for the Drosophila transcription factor SNAIL
TL;DR: Transient expression in co-transfection experiments using a SNA binding element (SBE) linked to a heterologous promoter indicates that SNA has the ability to function as a transcription activator.
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Estrogen receptor binding to a DNA response element in vitro is not dependent upon estradiol
TL;DR: It is found that estrogen does not significantly contribute to receptor-DNA complex formation and the ability of the ER to discriminate between target and nonspecific DNA sequences was also not dependent on the presence of estrogen.
Journal ArticleDOI
Ovarian steroid and cytokine modulation of human endometrial angiogenesis
Dan I. Lebovic,Jan L. Shifren,Isabelle P. Ryan,Michael D. Mueller,A. Korn,Philip D. Darney,Robert N. Taylor +6 more
TL;DR: It is proposed that regulation of endometrial angiogenesis is mediated indirectly, via steroid and cytokine actions on vascular endothelial growth factor (VEGF), and data is presented indicating that VEGF expression in normalendometrial stromal cells is increased by oestrogens and progestins.
Journal ArticleDOI
The Effects of Estrogen-Responsive Element- and Ligand-Induced Structural Changes on the Recruitment of Cofactors and Transcriptional Responses by ERα and ERβ
Ping Yi,Mark D. Driscoll,Jing Huang,Sumedha Bhagat,Russell Hilf,Robert A. Bambara,Mesut Muyan +6 more
TL;DR: It is shown here that ERα and ERβ bind to an ERE independently from ER ligands, and the ligand-dependent cofactor transcriptional intermediary factor-2, through a distinct surface, also interacts with ERα preferentially and independently of ligand.
Journal ArticleDOI
Enhancement of estrogen receptor transcriptional activity by the coactivator GRIP-1 highlights the role of activation function 2 in determining estrogen receptor pharmacology.
TL;DR: It is likely, therefore, that the C-terminal AF-2 domain may function as a molecular switch allowing the wild-type receptor to discriminate between agonists and antagonists as well as providing a surface with which associated proteins can interact.
References
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