A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
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Towards tailored assays for cell-based approaches to toxicity testing.
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Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE).
TL;DR: A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay, indicating that the binding protein is an ER-like protein.
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Transcriptional activation of DNA‐dependent protein kinase catalytic subunit gene expression by oestrogen receptor‐α
Senad Medunjanin,Sönke Weinert,David M. Poitz,Alexander Schmeisser,Ruth H. Strasser,Ruediger C. Braun-Dullaeus +5 more
TL;DR: E2‐induced DNA‐PK transactivation results in an increased ability of the cells to repair DNA DSB, which sheds new light on tumour biology and reveals new possibilities for the prevention and therapy of E2‐sensitive proliferative diseases.
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Different regions of the estrogen receptor are required for synergistic action with the glucocorticoid and progesterone receptors.
Andrew C.B. Cato,Helmut Ponta +1 more
TL;DR: It is concluded that the synergistic action of the receptors for estrogen and progesterone is mechanistically different from the synergism of the receptor for testosterone and glucocorticoid.
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Inhibitory effect of a novel non-steroidal aromatase inhibitor, YM511 on the proliferation of MCF-7 human breast cancer cell.
Masafumi Kudoh,Yoko Susaki,Yukitaka Ideyama,Taiki Nanya,Masamichi Mori,Hisataka Shikama,Takashi Fujikura +6 more
TL;DR: YM511 significantly inhibited testosterone-stimulated transcriptional activation of estrogen-responsive element (ERE) in MCF-7 cells transfected transiently with ERE-luciferase reporter plasmid, suggesting that the inhibition by YM511 of cell proliferation of MCf-7 is attributed to the decreased production of estrogen due to the inhibition of aromatase activity.
References
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