A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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TLDR
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
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Journal ArticleDOI
Functional Analysis of Promoter Region from Eel Cytochrome P450 1A1 Gene in Transgenic Medaka.
TL;DR: It is suggested that not only XRE but also other regulatory elements, possibly ERE, play an important role in induced and constitutive expressions of the eel CYP1A1 gene.
Journal ArticleDOI
Effects of estradiol and 4-hydroxytamoxifen on the conformation, thermal stability, and DNA recognition of estrogen receptor β
Veena Vijayanathan,Norma J. Greenfield,T. J. ThomasT.J. Thomas,Margarita M. Ivanova,Valentyn V. Tyulmenkov,Carolyn M. Klinge,Michael A. Gallo,Thresia Thomas +7 more
TL;DR: Coupled effects of estradiol, 4-hydroxytamoxifen, and the estrogen response element (ERE) on the helical content and thermal unfolding of ERβ demonstrate coupled effects of E...
Journal ArticleDOI
In vivo and in vitro protein-DNA interactions at the distal oestrogen response element of the chicken vitellogenin gene: Evidence for the sane protein binding to this sequence in hen and rooster liver
TL;DR: It is demonstrated that apart from the oestrogen receptor, at least one other protein can interact specifically with the chicken vitellogenin ERE, independently of hormonal expression of the gene.
Journal ArticleDOI
Potentiation of human estrogen receptor α-mediated gene expression by steroid receptor coactivator-1 (SRC-1) in Saccharomyces cerevisiae
TL;DR: The yeast Saccharomyces cerevisiae was used to reconstruct a human estrogen receptor alpha (ERalpha)-mediated transcription activation system and SRC-1 functions as a bona fide coactivator should permit a more thorough dissection of the factors involved in ERalpha-mediated transcriptional activation.
References
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Book
Gene Regulation by Steroid Hormones II
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TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
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TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?