A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
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Nuclear receptors, intestinal architecture and colon cancer: an intriguing link.
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TL;DR: The role of nuclear receptors in intestinal carcinogenesis is presented and their influence in maintenance of intestinal epithelium architecture is explored and nuclear receptors are explored as sensors of dietary and endogenous molecules, thus translating nutritional and hormonal stimuli into transcriptional modifications.
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Exon skipping gives rise to alternatively spliced forms of the estrogen receptor in breast tumor cells
TL;DR: Results of RNAse protection experiments which independently confirm the existence of these splicing variants in T47D cells and reveal the presence of variant ER transcripts, suggesting that they may be a common finding in tumor cell lines which express the estrogen receptor.
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Identification of a functional imperfect estrogen-responsive element in the 5'-promoter region of the human cathepsin D gene.
TL;DR: Identification of this second functional enhancer sequence in the 5'-promoter region of cathepsin D is consistent with the increasingly complex cell-specific regulation of hormone-responsive genes.
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Determinants contributing to estrogen-regulated expression of SK3.
TL;DR: The data suggest that estrogen regulates SK3 gene expression through interactions between ER alpha and Sp1, and only Sp1 specifically bound to the enhancer from Cos7 and L6 nuclear extracts.
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Production of a novel recombinant cell line for use as a bioassay system for detection of 2,3,7,8‐tetrachlorodibenzo‐P‐dioxin‐like chemicals
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References
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