A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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TLDR
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
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Journal ArticleDOI
Hamster estrogen receptor cDNA: cloning and mRNA expression.
Hari K. Bhat,Jaydutt V. Vadgama +1 more
TL;DR: Hamster estrogen receptor domain C (DNA binding domain) shows a 100% homology with a similar domain from mouse, rat, human, pig, sheep, horse and chicken estrogen receptor (Genebank reference ID: AF 181077).
Journal Article
High affinity binding of estrogen receptor to recombinant plasmids containing (dA-dC)n•(dG-dT)n sequences
Thresia Thomas,Thekkumkat Thomas +1 more
TL;DR: Results show that the presence of short stretches of (dA-dC)n.(dG-dT)n sequences has a dramatic effect in increasing the binding affinity of ER to DNA.
Estrogen Influences on the Developing Rat Hippocampus Through Interactions with the Brain Derived Neurotrophic Factor and P160 Coactivator Proteins
TL;DR: It is concluded, based on the immunocytochemical localization of ER, that the effects of estrogen on biochemistry and morphology of the developing hippocampus may be direct through the ER subtype in hippocampus.
Journal ArticleDOI
Binding of steroid receptors to the hres of mouse mammary tumor virus, chicken and xenopus vitellogenin and rabbit uteroglobin genes: Correlation with induction
TL;DR: The results suggest that events distal to DNA binding are essential for transcriptional activation, and that the function of the HRE is not simply to position the hormone receptor in the vicinity of the regulated promoter.
Journal Article
Ionic and Ligand-specific Effects on the DNA Binding of Progesterone Receptor Bound to the Synthetic Progestin R5020 and the Antiprogestin RU486
TL;DR: The results show thatPR.RU486 has lower binding affinity for specific DNA sequences than PR.R5020, but the binding affinity of both receptors is in a range that cannot preclude competitive interactions at the DNA recognition site, and the effects of Mg2+ and Ca2+ on PR binding to DNA further suggest that these cations could affect PR recognition of DNA in a ligand-specific manner.
References
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Gene Regulation by Steroid Hormones II
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TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
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TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?