A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
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TLDR
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.Abstract:
Sequences located upstream of the transcription initiation site of the Xenopus vitellogenin A2 (vit A2) gene contain a hormone dependent enhancer that confers estrogen control to the heterologous thymidine kinase (tk) promoter. As a minimal functional estrogen responsive element (ERE), we have defined the 13 bp palindrome GGTCACAGTGACC. This ERE binds estrogen receptor preferentially in vitro. Although the ERE shares some structural features with the glucocorticoid responsive element (GRE) it is distinct from this element since it neither binds glucocorticoid receptor in vitro nor does it confer glucocorticoid inducibility to a fusion gene. Point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.read more
Citations
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Journal ArticleDOI
Chromatin immunoprecipitation assay detects er recruitment to gene specific promoters in uterus
Sanhita Ray,Sanjoy K. Das +1 more
TL;DR: Examining estrogen-dependent transcriptional mechanism in respect of recruitment of estrogen receptor-alpha (ERα), a ligand-activated transcription factor, to uterine gene promoters in mice is examined.
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Estrogen Abolishes the Repression Role of gga-miR-221-5p Targeting ELOVL6 and SQLE to Promote Lipid Synthesis in Chicken Liver.
Dingding Zhang,Dandan Wang,Zhang Wang,Yang-Bin Wang,Guoxi Li,Guirong Sun,Yadong Tian,Ruili Han,Zhuanjian Li,Jiang Ruirui,Xiaojun Liu,Xiangtao Kang,Hong Li +12 more
TL;DR: The results showed that miR-221-5p was highly conserved among species, expressed in all tested tissues and significantly downregulated in peak-laying hen liver compared to pre-lays hen liver, indicating the highly conservative gga-miR- 221-5P could directly target ELOVL6 and SQLE mRNAs to affect the level of intracellular triglyceride and total cholesterol.
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Developmental changes in mullerian inhibiting substance in the cynomolgus monkey, Macaca fascicularis
Mary M. Lee,Michael L. Gustafson,Etsuji Ukiyama,Patricia K. Donahoe,David T. MacLaughlin,Michael Wexler,Hugh S. Keeping +6 more
TL;DR: It is confirmed that nonhuman primate and human MIS are highly homologous and have similar developmental patterns and expression was highest in the youngest animals and decreased progressively with increasing age.
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Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids.
Sayoko Ito-Harashima,Mami Matano,Kana Onishi,Tomofumi Nomura,Saki Nakajima,Shingo Ebata,Kazuhiro Shiizaki,Kazuhiro Shiizaki,Masanobu Kawanishi,Takashi Yagi +9 more
TL;DR: The yeast-based reporter gene assay will be valuable as a primary screening tool to detect and evaluate steroid hormone activities in various test samples and strongly support the detection of agonists, antagonists, and inverse agonists of steroid hormone receptors in the field of novel drug discovery and assessments of environmental pollutants.
Journal ArticleDOI
Bending of the estrogen response element by polyamines and estrogen receptors α and β: A fluorescence resonance energy transfer study
Veena Vijayanathan,Thekkumkat Thomas,Sandhya K. Nair,Akira Shirahata,Michael A. Gallo,Thresia Thomas +5 more
TL;DR: Polyamine-induced bending of the ERE might be a mechanism for enhancing ERα–ERE binding affinity and thereby fine-tuning the transcriptional response of estrogen-responsive genes.
References
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Book
Gene Regulation by Steroid Hormones II
Arun K. Roy,James H. Clark +1 more
TL;DR: The authors discuss the latest advances in molecular endocrinology: - steroid receptor binding to DNA sequences of hormonally controlled genes, - structure of genes controlled by steroid hormones, - heterogeneity of steroid receptors, - immunochemical approaches to receptor studies, and - the most recent approaches to steroid hormone action and biological response.
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TL;DR: Do you know the authors' friends become fans of molecular biology of egg maturation as the best book to read?